345852
Geranylgeranyltransferase I, GST-Fusion, His•Tag®, Rat, Recombinant, E. coli
Geranylgeranyltransferase I, GST-Fusion, His•Tag, Rat, Recombinant, E. coli, contains a 74 kDa α- & 43 kDa β-subunit. The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag.
Synonyme(s) :
GGTase I
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About This Item
Code UNSPSC :
12352202
Nomenclature NACRES :
NA.54
Produit recombinant
expressed in E. coli
Niveau de qualité
Pureté
≥90% (SDS-PAGE)
Forme
liquid
Fabricant/nom de marque
Calbiochem®
Conditions de stockage
OK to freeze
avoid repeated freeze/thaw cycles
Conditions d'expédition
wet ice
Température de stockage
−70°C
Description générale
Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved by TEV protease. Requires Zn2+ for optimal activity. The enzyme is purified with Zn2+ tightly bound, so addition of Zn2+ to the reaction mix is not necessary unless further manipulation of the enzyme results in release of the ions.
Recombinant, rat GGTase I composed of an α- and a β-subunit (74 and 43 kDa, respectively). The α-subunit is expressed as a GST-fusion construct with an N-terminal His•Tag sequence. Both the GST moiety and the His•Tag sequence can be utilized to facilitate enzyme capturing in prenylation and binding studies or, when desired, can also be cleaved off by TEV protease. Requires Zn2+ for optimal activity.
Actions biochimiques/physiologiques
1 pmol enzyme will transfer ≥3 pmol geranylgeranyl to RhoA in 10 min at 37°C, pH 7.2.
Avertissement
Toxicity: Standard Handling (A)
Forme physique
In 40 mM NaCl, 25 mM HEPES, 5 mM DTT, pH 7.2.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Autres remarques
Kalinin, A., et al. 2001. Protein Expr. Purif.22, 84.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.
Zhang, F.L., et al. 1994. J. Biol. Chem.269, 23465.
Moomaw, J.F., and Casey, P.J., 1992. J. Biol. Chem.267, 17438.
Informations légales
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Code de la classe de stockage
10 - Combustible liquids
Classe de danger pour l'eau (WGK)
WGK 1
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
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