06-562
Anti-erbB-2/HER-2 Antibody
Upstate®, from rabbit
Synonyme(s) :
CD340 antigen, CD340 antigen, NEU proto-oncogene, Tyrosine kinase-type cell surface receptor HER2, c-erb B2/neu protein, c-erb B2/neu protein, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog),
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About This Item
Code UNSPSC :
12352203
eCl@ss :
32160702
Nomenclature NACRES :
NA.41
Produits recommandés
Source biologique
rabbit
Niveau de qualité
Forme d'anticorps
purified antibody
Type de produit anticorps
primary antibodies
Clone
polyclonal
Espèces réactives
monkey, human, mouse
Réactivité de l'espèce (prédite par homologie)
rat
Fabricant/nom de marque
Upstate®
Technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotype
IgG
Numéro d'accès NCBI
Numéro d'accès UniProt
Conditions d'expédition
dry ice
Modification post-traductionnelle de la cible
unmodified
Informations sur le gène
human ... ERBB2(2064)
mouse ... Erbb2(13866)
rat ... Erbb2(24337)
rhesus monkey ... Erbb2(697573)
Description générale
The ErbB-2 proto-oncogene, also called Neu, EGFR-2 or HER-2, is a member of the transmembrane receptor tyrosine kinase family, which also includes EGF receptor and EGFR-3 (HER-3 or ErbB-3). Although, ErbB-2 does not have any known high-affinity ligands, its kinase activity can be activated without ligand by either overexpression or heteroassociation with other members of the ErbB family. Amplification of the ErbB-2 gene and overexpression of its product has been detected in almost 40% of primary human breast tumors, which correlates with poor prognosis in node positive breast cancer. ErbB-2 overexpression is also observed in ovarian, gastric, salivary and non-small cell lung carcinomas. Because of this, ErbB-2 is one of the major drug targets for breast cancer and other cancer treatments.
Spécificité
Recognizes c-erbB-2 (185 kDa), but not c-erbB-1(EGF-R), c-erbB-3 or c-erbB-4.
Immunogène
A KLH-linked peptide (AENPEYLGLDVPV) corresponding to aa 1243-1255 from the human c-erbB-2/HER-2/neu oncoprotein.
Application
Anti-erbB-2/HER-2 Antibody is an antibody against erbB-2/HER-2 for use in IH, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated erbB-2/HER-2 from 600 μg MCF-7 RIPA lysate, and previously in A431, LNCaP, and c-erbB-2 transfected 3T3/NIH RIPA lysate.
Immunohistochemistry:
A previous lot of antibody has been reported by an independent laboratory, to detect erbB-2/HER-2 in frozen and formalin/paraffin sections of rat breast.
4 μg of a previous lot immunoprecipitated erbB-2/HER-2 from 600 μg MCF-7 RIPA lysate, and previously in A431, LNCaP, and c-erbB-2 transfected 3T3/NIH RIPA lysate.
Immunohistochemistry:
A previous lot of antibody has been reported by an independent laboratory, to detect erbB-2/HER-2 in frozen and formalin/paraffin sections of rat breast.
Research Category
Signaling
Signaling
Research Sub Category
Growth Factors & Receptors
Growth Factors & Receptors
Qualité
Routinely evaluated by Western Blot on HEK293 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Her-2 on 10 μg of HEK293 lysates.
Western Blot Analysis:
1:500 dilution of this lot detected Her-2 on 10 μg of HEK293 lysates.
Description de la cible
185 kDa
Liaison
Replaces: 04-1127
Forme physique
Format: Purified
Protein A purified
Purified rabbit polyclonal IgG in buffer containing 10 mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Frozen solution.
Stockage et stabilité
Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Remarque sur l'analyse
Control
A431 cells, HEK293, Jurkat, MCF7 cell lysate.
A431 cells, HEK293, Jurkat, MCF7 cell lysate.
Autres remarques
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informations légales
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Clause de non-responsabilité
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Réf. du produit
Description
Tarif
Code de la classe de stockage
12 - Non Combustible Liquids
Classe de danger pour l'eau (WGK)
WGK 2
Point d'éclair (°F)
Not applicable
Point d'éclair (°C)
Not applicable
Certificats d'analyse (COA)
Recherchez un Certificats d'analyse (COA) en saisissant le numéro de lot du produit. Les numéros de lot figurent sur l'étiquette du produit après les mots "Lot" ou "Batch".
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Retrouvez la documentation relative aux produits que vous avez récemment achetés dans la Bibliothèque de documents.
Different mechanisms are implicated in ERBB2 gene overexpression in breast and in other cancers.
Vernimmen, D; Gueders, M; Pisvin, S; Delvenne, P; Winkler, R
British Journal of Cancer null
Introducing the cancer microenvironment section of Journal of Translational Medicine.
Fernando Vidal-Vanaclocha,Isaac P Witz
Journal of Translational Medicine null
Satomi Watanabe et al.
Cancer medicine, 8(3), 1258-1268 (2019-02-01)
HER2-targeted therapy, especially the anti-HER2 antibody trastuzumab, is standard for HER2-positive breast cancer; however, its efficacy is limited in a subpopulation of patients. HER3 ligand (heregulin)-dependent HER2-HER3 interactions play a critical role in the evasion of apoptosis and are therefore
Pathologic findings from the National Surgical Adjuvant Breast and Bowel Project: prognostic significance of erbB-2 protein overexpression in primary breast cancer.
Paik, S, et al.
Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 8, 103-112 (1990)
Increased expression of epidermal growth factor receptor induces sequestration of extracellular signal-related kinases and selective attenuation of specific epidermal growth factor-mediated signal transduction pathways.
Amyn A Habib, Soo Jin Chun, Benjamin G Neel, Timothy Vartanian
Molecular Cancer Research null
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