HPLC Analysis of Carotenoids by Normal Phase Chromatography on Ascentis® Si
CONDITIONS
column
Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase
[A] hexane; [B] ethyl acetate; [C] isopropanol; (85:10:5, A:B:C)
flow rate
1.0 mL/min
column temp.
30 °C
detector
UV, 450 nm
injection
10 μL
sample
50-100 μg/mL hexane
Description
Analysis Note
Ascentis silica is used in the analysis of naturally occurring carotenoid pigments in the form of alcohols, xanthophyll and astaxanthin.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany