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  • Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
ABSTRACT

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Toluene, anhydrous, 99.8%
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4-Methylmorpholine, purified by redistillation, ≥99.5%
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4-Methylmorpholine, purum, ≥98.0% (GC)
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4-Methylmorpholine, BioXtra, suitable for protein sequencing, ≥99.5% (GC)
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Benzyl alcohol, anhydrous, 99.8%
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N,N-Diisopropylethylamine, purified by redistillation, 99.5%
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Pyridine, ≥99%
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(3-Glycidyloxypropyl)trimethoxysilane, ≥98%
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Methanol-12C, 99.95 atom % 12C
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4-Methylmorpholine, ReagentPlus®, 99%
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Calcium chloride, AnhydroBeads, −10 mesh, ≥99.9% trace metals basis
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Benzyl alcohol, ≥99%, FCC, FG
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Calcium chloride, AnhydroBeads, −10 mesh, ≥99.99% trace metals basis
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Benzyl alcohol, natural, ≥98%, FG
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N,N-Diisopropylethylamine, ReagentPlus®, ≥99%
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Triphenyl phosphate solution, NMR reference standard, 3 mM in chloroform-d (99.8 atom % D), NMR tube size 5 mm × 8 in.
USP
Benzyl alcohol, United States Pharmacopeia (USP) Reference Standard
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Benzyl alcohol, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
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Methanol solution, NMR reference standard, 4% in methanol-d4 (99.8 atom % D), NMR tube size 3 mm × 8 in.
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Triphenyl phosphate solution, NMR reference standard, 0.0485 M in acetone-d6 (99.9 atom % D)
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Calcium chloride
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Ammonium hydroxide solution, BioUltra, ~1 M NH3 in H2O (T)
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