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Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Artificial cells, nanomedicine, and biotechnology (2013-06-26)

Ceren Erdem, Derya Koyuncu Zeybek, Gözde Aydoğdu, Bülent Zeybek, Sule Pekyardımcı, Esma Kılıç

ABSTRACT

In the present work, we designed an amperometric glucose biosensor based on nickel oxide nanoparticles (NiONPs)-modified carbon paste electrode. The biosensor was prepared by incorporation of glucose oxidase and NiONPs into a carbon paste matrix. It showed good analytical performances such as high sensitivity (367 μA mmolL(-1)) and a wide linear response from 1.9×10(-3) mmolL(-1) to 15.0 mmolL(-1) with a limit of detection (0.11 μmolL(-1)). The biosensor was used for the determination of glucose in human serum samples. The results illustrate that NiONPs have enormous potential in the construction of biosensor for determination of glucose.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

D-(+)-Glucose, suitable for mouse embryo cell culture, ≥99.5% (GC)

Sigma-Aldrich

Urea solution, 40 % (w/v) in H₂O

Sigma-Aldrich

D-(+)-Glucose, ≥99.5% (GC), BioXtra

Sigma-Aldrich

Urea-¹²C, 99.9 atom % ¹²C

Supelco

L-Ascorbic acid, analytical standard

Supelco

D-(+)-Glucose, analytical standard

Sigma-Aldrich

D-Glucose-¹²C₆, ¹⁶O₆, 99.9 atom % ¹⁶O, 99.9 atom % ¹²C

Sigma-Aldrich

L-Ascorbic acid, FCC, FG

Supelco

L-Ascorbic acid, certified reference material, TraceCERT^®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

Millipore

Urea solution, suitable for microbiology, 40% in H₂O

Sigma-Aldrich

Urea solution, BioUltra, ~8 M in H₂O

Sigma-Aldrich

D-(+)-Glucose, tested according to Ph. Eur.

Sigma-Aldrich

D-(+)-Glucose, BioUltra, anhydrous, ≥99.5% (sum of enantiomers, HPLC)

Sigma-Aldrich

D-(+)-Glucose, ACS reagent

Sigma-Aldrich

D-(+)-Glucose, ≥99.5% (GC)

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D-(+)-Glucose, Hybri-Max^™, powder, BioReagent, suitable for hybridoma

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Sodium phosphate monobasic dihydrate, purum p.a., crystallized, ≥99.0% (T)

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L-Ascorbic acid, suitable for cell culture, suitable for plant cell culture, ≥98%

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L-Ascorbic acid, meets USP testing specifications

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L-Ascorbic acid, powder, suitable for cell culture, γ-irradiated

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L-Ascorbic acid, reagent grade, crystalline

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L-Ascorbic acid, 99%

Sigma-Aldrich

L-Ascorbic acid, puriss. p.a., ≥99.0% (RT)

Sigma-Aldrich

L-Ascorbic acid, reagent grade

Sigma-Aldrich

L-Ascorbic acid, BioUltra, ≥99.5% (RT)

Sigma-Aldrich

L-Ascorbic acid, puriss. p.a., ACS reagent, reag. ISO, Ph. Eur., 99.7-100.5% (oxidimetric)

Sigma-Aldrich

L-Ascorbic acid, ACS reagent, ≥99%

Sigma-Aldrich

L-Ascorbic acid, BioXtra, ≥99.0%, crystalline

Sigma-Aldrich

L-Ascorbic acid, tested according to Ph. Eur.

Sigma-Aldrich

Sodium phosphate monobasic dihydrate, meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, 98-100.5% (calc. to the dried substance)