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Merck

Avanti lipid tools: connecting lipids, technology, and cell biology.

Biochimica et biophysica acta (2014-06-24)
Kacee H Sims, Ewan M Tytler, John Tipton, Kasey L Hill, Stephen W Burgess, Walter A Shaw
PMID24954118
ABSTRACT

Lipid research is challenging owing to the complexity and diversity of the lipidome. Here we review a set of experimental tools developed for the seasoned lipid researcher, as well as, those who are new to the field of lipid research. Novel tools for probing protein-lipid interactions, applications for lipid binding antibodies, enhanced systems for the cellular delivery of lipids, improved visualization of lipid membranes using gold-labeled lipids, and advances in mass spectrometric analysis techniques will be discussed. Because lipid mediators are known to participate in a host of signal transduction and trafficking pathways within the cell, a comprehensive lipid toolbox that aids the science of lipidomics research is essential to better understand the molecular mechanisms of interactions between cellular components. This article is part of a Special Issue entitled Tools to study lipid functions.

MATERIALS
Product Number
Brand
Product Description
265780
Sigma-Aldrich
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Gold, foil, thickness 0.025 mm, 99.99% trace metals basis
373184
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255734
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Gold coated glass cover slip, 99.999% (Au), diam. × thickness 15 mm × 130-170 μm, layer thickness 100 Å
643254
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Gold coated glass cover slip, 99.999% (Au), L × W × thickness 22 mm × 22 mm × 130-170 μm, square, layer thickness 100 Å
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Gold coated glass slide, layer thickness 1000 Å, 99.999% (Au)
643203
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Gold coated glass slide, layer thickness 100 Å, 99.999% (Au)
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Gold, tube, 50mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%
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Gold, tube, 50mm, outside diameter 1.0mm, inside diameter 0.7mm, wall thickness 0.15mm, as drawn, 99.95%
GF76009754
Gold, microfoil, disks, 10mm, thinness 0.25μm, specific density 483μg/cm2, permanent mylar 3.5μm support, 99.99+%
GF76961436
Gold, tube, 50mm, outside diameter 10.0mm, inside diameter 9.7mm, wall thickness 0.15mm, as drawn, 99.95%
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Gold, insulated wire, 2m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
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Gold, rod, 25mm, diameter 1.6mm, as drawn, 99.95%
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Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
GF86990350
Gold, tube, 100mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%
GF12231223
Gold, microleaf disks, 10mm, thinness 0.25μm, specific density 483μg/cm2, removable support, 99.99+%
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Gold, rod, 25mm, diameter 2.0mm, as drawn, 99.95%
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Gold, tube, 100mm, outside diameter 0.3mm, inside diameter 0.2mm, wall thickness 0.05mm, as drawn, 99.95%
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Gold, tube, 100mm, outside diameter 8mm, inside diameter 7.8mm, wall thickness 0.1mm, as drawn, 99.95%
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Gold, tube, 200mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%
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Gold, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
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Gold, rod, 10mm, diameter 5.0mm, as drawn, 99.95%
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Gold, insulated wire, 0.5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
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Gold, insulated wire, 0.2m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%