59140-U

SUPELCOSIL^™ LC-ABZ (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• Synonym(s): Amino-Benzene-Zone HPLC Column
• EC Number: 231-545-4
• UNSPSC Code: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

Properties

• product name: SUPELCOSIL^™ LC-ABZ HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• material: stainless steel column
• Agency: suitable for USP L60
• product line: SUPELCOSIL^™
• feature: endcapped
• manufacturer/tradename: SUPELCOSIL^™
• packaging: 1 ea of
• extent of labeling: 12.0% carbon loading
• parameter: ≤70 °C temp. limit; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 15 cm × 4.6 mm
• surface area: 170 m²/g
• surface coverage: surface coverage 3.4 μmol/m²
• matrix: silica gel, spherical particle platform; fully porous particle
• matrix active group: amide, alkyl phase
• particle size: 5 μm
• pore size: 120 Å
• pH range: 2-7.5
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

SUPELCOSIL LC-ABZ phase has a unique deactivation technology which provides excellent reversed-phase performance for basic compounds, as well as those that are acidic, polar neutral, and non-polar. Silanol suppressing practices such as using competing amines, operating at high ionic strength, and adjusting mobile phases to pH extremes are typically not required to obtain good peak shape on the LC-ABZ phase.

Application

• Validation of a high-performance liquid chromatography method for the assay of and determination of related organic impurities in nevirapine drug substance.: This paper validates a method using the SUPELCOSIL^™ LC-ABZ HPLC Column for assessing purity and impurities in HIV medication, showcasing the column′s high specificity and reproducibility (Li et al., 2000).


• A comparative study of the reversed-phase HPLC retention behaviour of S-adenosyl-L-methionine and its related metabolites on Hypersil ODS and Supelcosil LC-ABZ stationary phases.: This comparative study provides insights into the retention behaviors of bioactive compounds on SUPELCOSIL^™ LC-ABZ, affirming its versatility and robust performance in complex biochemical separations (Valkó et al., 1993).

Features and Benefits

• For acids, bases, zwitterions
• Increased polar retention relative to standard reversed-phases
• Unique selectivity
• Silanol deactivation
• Peak shape, efficiency, and retention are comparable to C8 phases for non-polar analytes

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Legal Information

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable