252-05N

Human Umbilical Artery Smooth Muscle Cells: HUASMC, neonatal

• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• biological source: human umbilicus (normal arteries)
• Quality Level: 100
• packaging: pkg of 500,000 cells
• manufacturer/tradename: Cell Applications, Inc
• growth mode: Adherent
• karyotype: 2n = 46
• morphology: smooth muscle
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): diabetes; stroke; cardiovascular diseases
• shipped in: dry ice
• storage temp.: −196°C

Description

General description

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HUASMC has been used to study effects of snake venom on smooth muscle cells (Mora, 2008); secretion mechanism of the (pro)renin receptor (Yoshikawa, 2011) and the role of proteasomes (Martin, 2014) in the development of cardiovascular diseases; and to develop novel material surfaces to inhibit smooth muscle cell proliferation at the same time allowing endothelial cells adhesion and proliferation in order to reduce risk associated with vascular implants (Kanie, 2012). Characterization: positive for smooth muscle cell specific alpha-actin expression.

Cell Line Origin

Artery

Application

vascular function, tissue engineering, pharmacology of vascular tissue

Components

Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO

Preparation Note

• 2nd passage, >500,000 cells in Smooth Muscle Cell Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the HUASMC Culture Protocol.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable