MABS1950
Anti-p15 Antibody, clone PAT56B
clone PAT56B, from rat
Synonym(s):
Cyclin-dependent kinase 4 inhibitor B, p14-INK4b, p15-INK4b
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
PAT56B, monoclonal
species reactivity
mouse
packaging
antibody small pack of 25 μg
technique(s)
immunohistochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Cdkn2B(12579)
General description
Cyclin-dependent kinase 4 inhibitor B (UniProt: P55271; also known as p14-INK4b, p15-INK4b) is encoded by the Cdkn2b gene (Gene ID: 12579) in murine species. p15-INK4b is a ubiquitously expressed member of the INK4 family of cyclin-dependent kinase inhibitors. It induces a G1-phase cell cycle arrest through heterodimerization with cyclin-dependent kinase 4/6, thereby serves as a tumor suppressor. It also serves as a potential effector of TGF-beta induced cell cycle arrest. p15 also plays an important role in the regulation of cellular commitment of hematopoietic progenitor cells and myeloid cell differentiation. It contains four ankyrin repeats (aa 5-34; 38-66; 71-100; and 104-130). p15 shares extensive homology with p16 and serves an important backup function for p16 in senescence. Cdkn2b gene is frequently hypermethylated in myeloid neoplasia. Genes coding for both p15 and p16 are reported to often deleted in human tumors. p15 can serve as a good prognostic marker for monitoring the response to treatment with DNA methylation inhibitors. (Ref.: Wolff, L., and Bies, J. (2013). Blood Cells Mol. Dis. 50(4); 227-231).
Specificity
Clone PAT56B is a rat monoclonal antibody that specifically detects murine Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).
Immunogen
GST/His-tagged recombinant full-length mouse Cyclin-dependent kinase 4 inhibitor B (p15-INK4b).
Application
Anti-p15, clone PAT56B, Cat. No. MABS1950, is a rat monoclonal antibody that detects Cyclin-dependent kinase 4 inhibitor B (p15-INK4b) and has been tested for use in Immunohistochemistry and Western Blotting.
Immunohistochemistry Analysis: A representative lot detected p15 in Immunohistochemistry applications (Munoz-Espin, D., et. al. (2013). Cell. 155(5):1104-18).
Research Category
Signaling
Signaling
Quality
Evaluated by Western Blotting in NIH/3T3 cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected p15 in NIH/3T3 cell lysates.
Western Blotting Analysis: 4 µg/mL of this antibody detected p15 in NIH/3T3 cell lysates.
Target description
~14 kDa observed; 13.79 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Daniel Muñoz-Espín et al.
Cell, 155(5), 1104-1118 (2013-11-19)
Cellular senescence disables proliferation in damaged cells, and it is relevant for cancer and aging. Here, we show that senescence occurs during mammalian embryonic development at multiple locations, including the mesonephros and the endolymphatic sac of the inner ear, which we
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