04-1578

Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11

clone 10D11, from mouse

• Synonym(s): Agammaglobulinaemia tyrosine kinase, B-cell progenitor kinase, Bruton agammaglobulinemia tyrosine kinase, Bruton tyrosine kinase, dominant-negative kinase-deficient Brutons tyrosine kinase, tyrosine-protein kinase BTK
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 10D11, monoclonal
• species reactivity: rat, human
• technique(s): immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1κ
• NCBI accession no.: NP_000052.1
• UniProt accession no.: Q06187
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... BTK(695)

Description

General description

Btk (Bruton’s tyrosine kinase also known as BPK or ATK) is a member of the TEC family of non-receptor protein tyrosine kinase which includes Bmx, Itk and TEC. BTK plays a crucial role in B cell activation and development. Similar to Etk-TNFR2 interaction, Btk interacts with Fas, a member of the TNFR family involved in apoptosis in a ligand-independent manner. Mutations in the Btk gene have been linked to severe developmental blocks in human B-cell ontogeny leading to X-linked agammaglobulinemia (XLA).

Specificity

Recognizes human Btk, MW ~77 kDa.
A higher molecular weight protein may be detected, which is thought to be a phosphorylated species of Btk or a related protein.

Immunogen

Epitope: Intracellular

GST-tagged recombinant protein corres-ponding to human Btk.

Application

Detect Bruton′s tyrosine kinase (Btk) using this Anti-Bruton′s tyrosine kinase (Btk) Antibody, clone 10D11 validated for use in WB & IP.

Immunoprecipitation Analysis: A previous lot of this antibody has been reported by an independent laboratory to immunoprecipitate Btk in the presence of 0.5% SDS. Not recommended for IP/kinase assays.

Research Category
Inflammation & Immunology

Research Sub Category
Immunological Signaling

Quality

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.05- 1 µg/mL of this antibody detected Btk on 10 µg of Raji cell lysate.

Target description

~77 kDa

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Raji cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable