SHC004H
MISSION® pLKO.1-puro TurboGFP™ shRNA Control Transduction Particles, High Titer
High Titer, shRNA sequence targeting tGFP
Synonym(s):
MISSION®, MISSION® Control Transduction Particles
About This Item
Recommended Products
Quality Level
product line
MISSION®
concentration
≥1x109 VP/ml (via p24 assay)
technique(s)
capture ELISA: 109 TU/mL using p24
shipped in
dry ice
storage temp.
−70°C
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1 of 4
This Item | SHC003V | SHC003H | SHC004V |
---|---|---|---|
MISSION® | MISSION® | MISSION® | MISSION® |
capture ELISA: 109 TU/mL using p24 | capture ELISA: 106 TU/mL using p24 | capture ELISA: 109 TU/mL using p24 | capture ELISA: 106 TU/mL using p24 |
−70°C | −70°C | −70°C | −70°C |
dry ice | dry ice | dry ice | dry ice |
General description
The TurboGFP shRNA control transduction particles are produced from the sequence-verified lentiviral plasmid, pLKO.1-puro-TurboGFP shRNA (SHC004). Self-inactivating replication incompetent viral particles are produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. In addition, the Control Transduction Particles are pseudotyped with an envelope G glycoprotein from Vesicular Stomatitis Virus (VSV-G), allowing transduction of a wide variety of mammalian cells. 200 μl of 109 TU/ml (via p24 titering assay) lentiviral particles are provided as frozen stock.
This high titer offering is recommended for customers: who need to utilize smaller volumes of virus, who have very difficult to transduce cell lines, or who need to transduce large numbers of cells.
To see more application data, protocols, vector maps visit sigma.com/shrna.
Application
Components
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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