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LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18

LC/MS Analysis of Vitamin D2, D3, Gamma- and Alpha-Tocopherol on Ascentis® Express C18 application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® Express C18, 2,7 μm HPLC-Säule

2.7 μm particle size, L × I.D. 10 cm × 2.1 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

(+)-α-Tocopherol

from vegetable oil, Type V, ~1000 IU/g

Vitamin D3 solution

100 μg/mL in ethanol, 97% (CP)

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)

mobile phase

[A] water; [B] acetonitrile; (5:95, A:B)

flow rate

0.4 mL/min

column temp.

40 °C

detector

MS, ESI+

injection

2 μL

sample

1 μg/mL in acetonitrile

Beschreibung

Hinweis zur Analyse

Analysis was conducted using standard solution, detection on LC/MS system.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany