HPLC Analysis of Tramadol Enantiomers on Astec® CYCLOBOND I 2000 DMP
CONDITIONS
column
CYCLOBOND I 2000 DMP, 25 cm x 4.6 mm I.D., 5 μm particles (20724AST)
mobile phase
[A] 0.1% triethylamine acetate, pH 5.0; [B] acetonitrile; (80:20, A:B)
flow rate
1.2 mL/min
column temp.
23 °C
detector
UV, 254 nm
injection
5 μL
sample
5 mg/mL in methanol
Beschreibung
Rechtliche Hinweise
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
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