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HPLC Analysis of Proteins on BIOshell A160 Peptide C18

HPLC Analysis of Proteins on BIOshell A160 Peptide C18 application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

53307-U

Ascentis® Express Peptid ES-C18, 2,7 μm HPLC-Säule

2.7 μm particle size, L × I.D. 15 cm × 2.1 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

T9128

Trypsin-Inhibitor aus Glycine max (soybean)

lyophilized powder

A2327

Adrenomedullin 52 vom Menschen

≥90% (HPLC), powder

R6513

Ribonuklease A aus Rinderpankreas

for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

S7571

Superoxid-Dismutase aus Rindererythrocyten

lyophilized powder, ≥3,000 units/mg protein, Protein ≥95 % by biuret

41235

Ovalbumin-Atto 488-Konjugat

suitable for fluorescence

C6749

Cytochrome c equine

recombinant, expressed in E. coli

C2624

Carboanhydrase aus Rindererythrocyten

≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder

CONDITIONS

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile

gradient

held at 0% B for 0.5 min; 0 to 100% B in 7 min

flow rate

0.3 mL/min

pressure

4743 psi (327 bar)

column temp.

35 °C

detector

UV, 220 nm

injection

1 μL

sample

0.2 - 0.6 g/L in mobile phase A

Beschreibung

Rechtliche Hinweise

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