HPLC Analysis of mAb on Antibodix^® WCX-NP5, 25 cm Columns

column

Antibodix WCX-NP5, 25 cm x 4.6 mm I.D., 5 μm particles (Z777294)

mobile phase

(A) 10 mM sodium phosphate buffer, pH 7.5; (B) A + 100 mM NaCl

gradient

15 to 55% B in 30 min

flow rate

0.8 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

10 μL

sample

monoclonal antibody