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HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express C18

HPLC Analysis of Diet Soda Spiked with Neotame on Ascentis® Express C18 application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

Ascentis® Express C18, 2,7 μm HPLC-Säule

2.7 μm particle size, L × I.D. 3 cm × 4.6 mm

CONDITIONS

column

Ascentis Express C18, 3 cm x 4.6 mm I.D., 2.7 μm particles (53818-U)

mobile phase

[A] 100 mM ammonium acetate, pH 5.6 (titrated with acetic acid); [B] water; [C] acetonitrile

gradient

20% A constant; 75 to 20% B, 5 to 60% C in 1 min; held at 20% B, 60% C for 0.1 min

flow rate

3 mL/min

pressure

3191 psi (220 bar)

column temp.

40 °C

detector

UV, 214 nm

injection

1 μL

sample

neotame 350 μg/mL in diet cola

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of common drink additives used in diet soda.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany