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HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection)

HPLC Analysis of an Energy Drink on Ascentis® Express RP-Amide (UV detection) application for HPLC

Materialien

Analytische Säule

Produkt-Nr.
Beschreibung
Preisangaben

53916-U

Ascentis® Express RP-Amid, 2,7 μm HPLC-Säule

2.7 μm particle size, L × I.D. 5 cm × 3 mm

Standard

Produkt-Nr.
Beschreibung
Preisangaben

C1778

Koffein

Sigma Reference Standard, vial of 250 mg

S1626

Sorbinsäure

99.0-101.0% anhydrous basis

47862

Pyridoxinhydrochlorid (B6)

analytical standard

47865-U

Nicotinamid (Niacinamid)

analytical standard

47861

Riboflavin (B2)

analytical standard

8S61336

SS Benzoesäure

2000 μg/mL in dichloromethane, analytical standard

CONDITIONS

column

Ascentis Express RP-Amide, 5 cm x 3 mm I.D., 2.7 μm particles (53916-U)

mobile phase

[A] water with 0.1% TFA; [B] acetonitrile with 0.1% TFA

flow rate

0.6 mL/min

pressure

1146 psi (79 bar)

column temp.

35 °C

detector

UV, 254 nm

injection

2 μL

sample

Energy Drink Diluted 1:9 in acetonitrile

Beschreibung

Hinweis zur Analyse

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of common drink additives used in caffeinated energy drinks.

Rechtliche Hinweise

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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