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Initiation of DNA replication requires actin dynamics and formin activity.

The EMBO journal (2017-10-07)
Nikolaos Parisis, Liliana Krasinska, Bethany Harker, Serge Urbach, Michel Rossignol, Alain Camasses, James Dewar, Nathalie Morin, Daniel Fisher
ZUSAMMENFASSUNG

Nuclear actin regulates transcriptional programmes in a manner dependent on its levels and polymerisation state. This dynamics is determined by the balance of nucleocytoplasmic shuttling, formin- and redox-dependent filament polymerisation. Here, using

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Anti-Aktin, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Latrunculin A, from sea sponge, ≥85% (HPLC), waxy solid
Sigma-Aldrich
Anti-PCNA (Ab-1) Mouse mAb (PC10), liquid, clone PC10, Calbiochem®
Sigma-Aldrich
Monoclonal Anti-PSTAIR antibody produced in mouse, clone PSTAIR, ascites fluid