- Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.
Increased intracellular pH is necessary for adult epithelial and embryonic stem cell differentiation.
The Journal of cell biology (2016-11-09)
Bryne Ulmschneider, Bree K Grillo-Hill, Marimar Benitez, Dinara R Azimova, Diane L Barber, Todd G Nystul
PMID27821494
ZUSAMMENFASSUNG
Despite extensive knowledge about the transcriptional regulation of stem cell differentiation, less is known about the role of dynamic cytosolic cues. We report that an increase in intracellular pH (pHi) is necessary for the efficient differentiation of Drosophila adult follicle stem cells (FSCs) and mouse embryonic stem cells (mESCs). We show that pHi increases with differentiation from FSCs to prefollicle cells (pFCs) and follicle cells. Loss of the Drosophila Na
MATERIALIEN
Produktnummer
Marke
Produktbeschreibung
Sigma-Aldrich
Albumin -Lösung, 35% in DPBS, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
Dulbecco′s Modified Eagle′s Medium, Without glucose, L-glutamine, phenol red, sodium pyruvate and sodium bicarbonate, powder, suitable for cell culture