A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.

Macromolecular bioscience (2015-07-03)

Fabio Salvatore Palumbo, Antonella Bavuso Volpe, Flavia Bongiovì, Giovanna Pitarresi, Gaetano Giammona

ZUSAMMENFASSUNG

Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

D2650

Sigma-Aldrich

Dimethylsulfoxid, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

179124

Sigma-Aldrich

Aceton, ACS reagent, ≥99.5%

472301

Sigma-Aldrich

Dimethylsulfoxid, ACS reagent, ≥99.9%

151882

Sigma-Aldrich

Deuteriumoxid, 99.9 atom % D

D8418

Sigma-Aldrich

Dimethylsulfoxid, for molecular biology

D2438

Sigma-Aldrich

Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

D5879

Sigma-Aldrich

Dimethylsulfoxid, ReagentPlus^®, ≥99.5%

319929

Sigma-Aldrich

1,2-Dichlorethan, ACS reagent, ≥99.0%

179973

Sigma-Aldrich

Aceton, Laboratory Reagent, ≥99.5%

D4540

Sigma-Aldrich

Dimethylsulfoxid, ≥99.5% (GC), suitable for plant cell culture

41640

Sigma-Aldrich

Dimethylsulfoxid, puriss. p.a., ACS reagent, ≥99.9% (GC)

130672

Sigma-Aldrich

N-Hydroxysuccinimid, 98%

G8540

Sigma-Aldrich

L-Glutamin, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source

32201

Sigma-Aldrich

Aceton, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

450510

Sigma-Aldrich

Deuteriumoxid, 99.9 atom % D, contains 0.05 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

G3126

Sigma-Aldrich

L-Glutamin, ReagentPlus^®, ≥99% (HPLC)

41639

Sigma-Aldrich

Dimethylsulfoxid, BioUltra, for molecular biology, ≥99.5% (GC)

320110

Sigma-Aldrich

Aceton, ACS reagent, ≥99.5%

G5792

SAFC

L-Glutamin

03449

Sigma-Aldrich

N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimid -hydrochlorid, ≥99.0% (AT)

534064

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Aceton, histological grade, ≥99.5%

221775

Sigma-Aldrich

Kupfer(II)-bromid, 99%

D9170

Sigma-Aldrich

Dimethylsulfoxid, PCR Reagent

293040

Sigma-Aldrich

Deuteriumoxid, 99.9 atom % D, contains 0.75 wt. % 3-(trimethylsilyl)propionic-2,2,3,3-d₄ acid, sodium salt

49419

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L-Glutamin, BioUltra, ≥99.5% (NT)

161691

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Bis(4-nitrophenyl)carbonat, ≥99%

34943

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Dimethylsulfoxid, puriss. p.a., dried, ≤0.02% water

756822

Sigma-Aldrich

Deuteriumoxid, filtered, 99.8 atom % D

24201

Sigma-Aldrich

Aceton, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99% (GC)

391085

Sigma-Aldrich

Ethylendiamin, purified by redistillation, ≥99.5%