Direkt zum Inhalt
Merck
  • Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Electrochemical glucose biosensor based on nickel oxide nanoparticle-modified carbon paste electrode.

Artificial cells, nanomedicine, and biotechnology (2013-06-26)
Ceren Erdem, Derya Koyuncu Zeybek, Gözde Aydoğdu, Bülent Zeybek, Sule Pekyardımcı, Esma Kılıç
ZUSAMMENFASSUNG

In the present work, we designed an amperometric glucose biosensor based on nickel oxide nanoparticles (NiONPs)-modified carbon paste electrode. The biosensor was prepared by incorporation of glucose oxidase and NiONPs into a carbon paste matrix. It showed good analytical performances such as high sensitivity (367 μA mmolL(-1)) and a wide linear response from 1.9×10(-3) mmolL(-1) to 15.0 mmolL(-1) with a limit of detection (0.11 μmolL(-1)). The biosensor was used for the determination of glucose in human serum samples. The results illustrate that NiONPs have enormous potential in the construction of biosensor for determination of glucose.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Natriumphosphat Heptahydrat, ACS reagent, 98.0-102.0%
Sigma-Aldrich
D-(+)-Glukose, ≥99.5% (GC)
Sigma-Aldrich
D-(+)-Glukose, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%
Sigma-Aldrich
Harnstoff, powder, BioReagent, for molecular biology, suitable for cell culture
Sigma-Aldrich
L-Ascorbinsäure, powder, suitable for cell culture, γ-irradiated
Sigma-Aldrich
L-Ascorbinsäure, BioXtra, ≥99.0%, crystalline
Sigma-Aldrich
Natriumphosphat, einbasisch Dihydrat, 98-100.5% (calc. to the dried substance), meets analytical specification of Ph. Eur., BP, USP, FCC, E 339
Sigma-Aldrich
Dextrose, 97.5-102.0% anhydrous basis, meets EP, BP, JP, USP testing specifications
Sigma-Aldrich
L-Ascorbinsäure, suitable for cell culture, suitable for plant cell culture, ≥98%
Sigma-Aldrich
Harnstoff, ACS reagent, 99.0-100.5%
Sigma-Aldrich
Harnstoff -Lösung, BioUltra, ~8 M in H2O
Sigma-Aldrich
D-(+)-Glukose, ≥99.5% (GC), BioXtra
Supelco
Harnstoff, 8 M (after reconstitution with 16 mL high purity water)
Supelco
Ascorbinsäure, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Harnsäure, ≥99%, crystalline
Sigma-Aldrich
Natriumphosphat, einbasisch Dihydrat, BioUltra, for molecular biology, ≥99.0% (T)
Supelco
Dextrose, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
L-Ascorbinsäure, reagent grade, crystalline
USP
Dextrose, United States Pharmacopeia (USP) Reference Standard