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A DNA-based molecular probe for optically reporting cellular traction forces.

A DNA-based molecular probe for optically reporting cellular traction forces.

Nature methods (2014-10-13)

Brandon L Blakely, Christoph E Dumelin, Britta Trappmann, Lynn M McGregor, Colin K Choi, Peter C Anthony, Van K Duesterberg, Brendon M Baker, Steven M Block, David R Liu, Christopher S Chen

ZUSAMMENFASSUNG

We developed molecular tension probes (TPs) that report traction forces of adherent cells with high spatial resolution, can in principle be linked to virtually any surface, and obviate monitoring deformations of elastic substrates. TPs consist of DNA hairpins conjugated to fluorophore-quencher pairs that unfold and fluoresce when subjected to specific forces. We applied TPs to reveal that cellular traction forces are heterogeneous within focal adhesions and localized at their distal edges.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

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Acetonitril, suitable for HPLC, gradient grade, ≥99.9%

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Methanol, suitable for HPLC, ≥99.9%

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Ethyl alcohol, Pure 200 proof, for molecular biology

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Dimethylsulfoxid, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

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Dimethylsulfoxid, ACS reagent, ≥99.9%

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Trifluoressigsäure, ReagentPlus^®, 99%

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Trifluoressigsäure, suitable for HPLC, ≥99.0%

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Dimethylsulfoxid, for molecular biology

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Methanol, ACS reagent, ≥99.8%

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Ethyl alcohol, Pure, 200 proof, ACS reagent, ≥99.5%

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Essigsäure, glacial, ACS reagent, ≥99.7%

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Dimethylsulfoxid, suitable for HPLC, ≥99.7%

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Acetonitril, HPLC Plus, ≥99.9%

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Dimethylsulfoxid, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

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Methanol, suitable for HPLC, gradient grade, ≥99.9%

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Essigsäure, glacial, ReagentPlus^®, ≥99%

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Dimethylsulfoxid, ReagentPlus^®, ≥99.5%

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Methanol, HPLC Plus, ≥99.9%

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Ethyl alcohol, Pure, 200 proof, meets USP testing specifications

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Trifluoressigsäure, puriss. p.a., suitable for HPLC, ≥99.0% (GC)

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Dimethylsulfoxid, ≥99.5% (GC), suitable for plant cell culture

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Acetonitril, ACS reagent, ≥99.5%

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Formamid, ReagentPlus^®, ≥99.0% (GC)

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Acetonitril, ≥99.9% (GC)

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Dimethylsulfoxid, puriss. p.a., ACS reagent, ≥99.9% (GC)

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Magnesiumchlorid -Lösung, for molecular biology, 1.00 M±0.01 M

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Harnstoff, powder, BioReagent, for molecular biology, suitable for cell culture

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DL-Dithiothreitol -Lösung, BioUltra, for molecular biology, ~1 M in H₂O