Direkt zum Inhalt
Merck
  • Synthesis of poly(meth)acrylates with thioether and tertiary sulfonium groups by ARGET ATRP and their use as siRNA delivery agents.

Synthesis of poly(meth)acrylates with thioether and tertiary sulfonium groups by ARGET ATRP and their use as siRNA delivery agents.

Biomacromolecules (2014-12-18)
Matthew C Mackenzie, Arun R Shrivats, Dominik Konkolewicz, Saadyah E Averick, Michael C McDermott, Jeffrey O Hollinger, Krzysztof Matyjaszewski
ZUSAMMENFASSUNG

The field of RNA interference depends on the development of safe and efficient carriers for short interfering ribonucleic acid (siRNA) delivery. Conventional cationic monomers for siRNA delivery have utilized the nitrogen heteroatom to produce cationic charges. Here, we polymerized cationic sulfonium (meth)acrylate by activators regenerated by electron transfer (ARGET) atom transfer radical polymerization (ATRP) to form polymers with narrow molecular weight distributions for siRNA delivery. The tertiary sulfonium species was stable toward dealkylation in water but less stable in the polar aprotic solvent dimethyl sulfoxide. Block copolymers poly(ethylene oxide) with poly(meth)acrylate containing sulfonium moieties were prepared as an siRNA delivery platform. Results suggested block copolymers were biocompatible up to 50 μg/mL in vitro and formed polyplexes with siRNA. Additionally, block copolymers protected siRNAs against endonuclease digestion and facilitated knockdown of glyceraldehyde 3-phosphate dehydrogenase (Gapdh) mRNA expression in murine calvarial preosteoblasts. The versatility, biocompatibility, and cationic nature of these tertiary sulfonium groups are expected to find widespread biological applications.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Methanol, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Aceton, ACS reagent, ≥99.5%
Sigma-Aldrich
Salzsäure, ACS reagent, 37%
Sigma-Aldrich
Methanol, ACS reagent, ≥99.8%
Sigma-Aldrich
Aceton, suitable for HPLC, ≥99.9%
Sigma-Aldrich
N,N-Dimethylformamid, ACS reagent, ≥99.8%
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, ≥99.9%
Sigma-Aldrich
N,N-Dimethylformamid, suitable for HPLC, ≥99.9%
Sigma-Aldrich
Salzsäure, ACS reagent, 37%
Sigma-Aldrich
Aceton, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Methanol, HPLC Plus, ≥99.9%
Sigma-Aldrich
Magnesiumsulfat, anhydrous, ReagentPlus®, ≥99.5%
Sigma-Aldrich
Chlorwasserstoff -Lösung, 4.0 M in dioxane
Sigma-Aldrich
Aluminiumoxid, activated, basic, Brockmann I
Sigma-Aldrich
Aceton, Laboratory Reagent, ≥99.5%
Sigma-Aldrich
Salzsäure -Lösung, 1.0 N, BioReagent, suitable for cell culture
Sigma-Aldrich
Magnesiumsulfat, anhydrous, reagent grade, ≥97%
Sigma-Aldrich
L-Ascorbinsäure, powder, suitable for cell culture, γ-irradiated
Sigma-Aldrich
L-Ascorbinsäure, BioXtra, ≥99.0%, crystalline
Sigma-Aldrich
Acrylsäure, anhydrous, contains 200 ppm MEHQ as inhibitor, 99%
Sigma-Aldrich
Methanol, suitable for HPLC, gradient grade, suitable as ACS-grade LC reagent, ≥99.9%
Sigma-Aldrich
Aluminiumoxid, activated, neutral, Brockmann I
Sigma-Aldrich
4-(Dimethylamino)-pyridin, ReagentPlus®, ≥99%
Sigma-Aldrich
Salzsäure, meets analytical specification of Ph. Eur., BP, NF, fuming, 36.5-38%
Sigma-Aldrich
Salzsäure, 37 wt. % in H2O, 99.999% trace metals basis
Sigma-Aldrich
Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)
Sigma-Aldrich
N,N-Dimethylformamid, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)
Sigma-Aldrich
Salzsäure, 36.5-38.0%, BioReagent, for molecular biology
Sigma-Aldrich
Chlorwasserstoff -Lösung, 2.0 M in diethyl ether
Sigma-Aldrich
Salzsäure, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., fuming, ≥37%, APHA: ≤10