N-Terminal signal sequence is required for cellular trafficking and hyaluronan-depolymerization of KIAA1199.

N-Terminal signal sequence is required for cellular trafficking and hyaluronan-depolymerization of KIAA1199.

FEBS letters (2013-11-26)

Hiroyuki Yoshida, Aya Nagaoka, Sachiko Nakamura, Megumi Tobiishi, Yoshinori Sugiyama, Shintaro Inoue

ZUSAMMENFASSUNG

Recently, we disclosed that KIAA1199-mediated hyaluronan (HA) depolymerization requires an acidic cellular microenvironment (e.g. clathrin-coated vesicles or early endosomes), but no information about the structural basis underlying the cellular targeting and functional modification of KIAA1199 was available. Here, we show that the cleavage of N-terminal 30 amino acids occurs in functionally matured KIAA1199, and the deletion of the N-terminal portion results in altered intracellular trafficking of the molecule and loss of cellular HA depolymerization. These results suggest that the N-terminal portion of KIAA1199 functions as a cleavable signal sequence required for proper KIAA1199 translocation and KIAA1199-mediated HA depolymerization.

MATERIALIEN

Produktnummer

Marke

Produktbeschreibung

53747

Sigma-Aldrich

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H5388

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PNGase F aus Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE), for proteomics

H7630

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Hyaluronsäure Natriumsalz aus Rinderaugen-Glaskörper

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Hyaluronsäure Natriumsalz aus Streptococcus equi sp., mol wt 1,000,000-1,250,000

40583

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Hyaluronsäure Natriumsalz aus Streptococcus equi sp., mol wt 8,000-15,000

G1549

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PNGase F aus Elizabethkingia meningoseptica, ready-to-use solution, recombinant, expressed in E. coli

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Hyaluronsäure Natriumsalz aus Streptococcus equi sp., mol wt 300,000-500,000

42686

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Hyaluronsäure Natriumsalz aus Streptococcus equi sp., mol wt 30,000-50,000

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94137

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