GE17-6001-14
Immobiline® DryStrip pH 3-10
Cytiva 17-6001-14, pack of 13 cm
About This Item
Empfohlene Produkte
Form
solid
Leistungsmerkmale
wetted part: no
Verpackung
pack of 13 cm
Hersteller/Markenname
Cytiva 17-6001-14
Farbe
Colorless
Löslichkeit
cold water: soluble (Insoluble in cold and hot water)
Versandbedingung
dry ice
Lagertemp.
−20°C
Allgemeine Beschreibung
Immobiline® DryStrip gels are available in fourteen overlapping pH ranges and five strip lengths (7, 11, 13, 18, and 24 cm) and represent the most comprehensive selection of narrow-, medium-, and wide-range IPG strips available. The five narrow pH range (1 pH unit), 24-cm IPG strips deliver the highest resolution and protein loading capacity available, which results in well separated spots with high protein content after the second-dimension separation.
The pH 3–11 NL strip covers most proteins found in prokaryotic and eukaryotic cells while the narrow-range strips can be used when higher resolution is required. The pH gradients at the extreme ends of the pH scale are nonlinear to distribute the proteins evenly over the gel length to obtain maximal resolution.
Immobiline® DryStrip gels are rehydrated with a matching IPG Buffer prior to use. Samples can be included directly in the rehydration solution for efficient loading of dilute samples or for loading larger amounts of protein. Samples can also be loaded after rehydration. The first-dimension separation is carried out using Ettan™ IPGphor 3 IEF system or Multiphor II flatbed system.
Anwendung
Leistungsmerkmale und Vorteile
- Maximize resolution, loading capacity, and reproducibility of first-dimension isoelectric focusing (IEF).
- Choose from a comprehensive range of overlapping Immobiline® DryStrip gels that cover narrow (1 pH unit), medium (3–5 pH units), and broad (8 pH units) pH ranges.
- Select the broad pH 3–11 NL gradient Immobiline® DryStrip gel for fast and efficient screening to gain a broad overview of total protein distribution.
- For more detailed studies or when using greater protein loads, choose from four new overlapping strips covering the pH 3 to 11 range.
- Higher resolution can be obtained using narrow-range (1 pH unit) Immobiline® DryStrip gels.
- Use matching IPG buffers for optimized running conditions.
- Use IPG strips directly after reswelling without further manipulation.
- Barcoded strips for convenient sample and batch tracking.
Lagerung und Haltbarkeit
Hinweis zur Analyse
Rechtliche Hinweise
Lagerklassenschlüssel
13 - Non Combustible Solids
WGK
WGK 2
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
This page describes troubleshooting second-dimension SDS-PAGE with Multiphor™ II Electrophoresis System.
Protokolle
2-D Clean-Up Kit from GE Healthcare is designed to prepare samples that would otherwise produce poor 2-D results.
This page describes troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from GE Healthcare.
Rehydration Solutions
This page describes two-dimensional separation of protein samples with GE Healthcare products.
Verwandter Inhalt
This page shows how to apply equilibrated Immobiline DryStrip gels
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