CLLS1199

MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-)

human female maMilliporeary glands (Source Disease: Fibrocystic Disease)

• UNSPSC-Code: 41106514
• NACRES: NA.81

Eigenschaften

• product name: MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-),
• Biologische Quelle: human female mammary glands (Source Disease: Fibrocystic Disease)
• OMIM-Hinterlegungsnummer: 171834; 601728
• Lagertemp.: −196°C
• Angaben zum Gen: human ... PIK3CA(5290) , PTEN(5728)

Beschreibung

Allgemeine Beschreibung

MCF10A CELLS PTEN (-/-) PIK3CA H1047R (+/-) are pre-neoplastic mammary epithelial cells from a human adult female with ZFN modifications.

This cell line was derived from ATCC Catalog No. CRL-10317.

Anwendung

Media Renewal changes two to three times per week.

Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.

Subculture Ratio: approx. 1:3-1:8

The base medium for this cell line is Dulbecco′s Modified Eagle′s Medium/Ham′s Nutrient Mixture F-12, Cat. No. 51448C. To make the complete growth medium, add the following components to the base medium: horse serum, Cat. No. H1270, to a final concentration (v/v) of 5%; cholera toxin, Cat. No. C8052, to a final concentration of 1 ng/mL; human insulin, Cat. No. I9278, to a final concentration of 10 ug/mL; epidermal growth factor, Cat. No. E9644, to a final concentration of 10 ng/mL; and hydrocortisone, Cat. No. H6909, to a final concentration of 0.5 ug/mL.

Cell freezing medium - DMSO 1X, Cat. No. C6164.

Leistungsmerkmale und Vorteile

These MCF10A cells are adherent with a doubling time of approx. 20 hours

Zinc finger nuclease (ZFN) modifications on chromosomes 10q23.3 and 3q26.3

Qualität

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation.

Haftungsausschluss

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit-Komponenten auch einzeln erhältlich

• MCF10A CONTROL CELLS 1 vial

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable