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Dokumente

A5713

Monoklonaler Anti-polyhistidin-Antikörper

Name: Monoklonaler Anti-polyhistidin-Antikörper
Brand: SIGMA

clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins

Synonym(e):

Monoklonales 6-His-Epitop-Tag, Monoklonales 6xHis-Tag, Monoklonales HHHHHH-Epitop-Tag, Monoklonales Hexahistidin-Tag, Monoklonales His-Tag, Monoklonales His6-Tag, Monoklonales Histidin-Tag, Monoklonales Polyhistidin-Tag

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About This Item

UNSPSC-Code:

12352203

NACRES:

NA.56

Biologische Quelle

mouse

Konjugat

agarose conjugate

Antikörperform

purified immunoglobulin

Antikörper-Produkttyp

primary antibodies

Klon

HIS-1, monoclonal

Form

PBS suspension

Isotyp

IgG2a

Kapazität

15-30 nmol/mL binding capacity

Eignung

suitable for purification of HIS tagged recombinant proteins

Versandbedingung

wet ice

Lagertemp.

2-8°C

Posttranslationale Modifikation Target

unmodified

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Allgemeine Beschreibung

This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.

Spezifität

Der Antikörper erkennt synthetisches sowie natives oder denaturiertes Polyhistidin und reduzierte Formen von mit 6X Histidinen markierten Proteinen, die in ausgewählten Vektoren exprimiert werden.

Immunogen

Rekombinantes, mit Polyhistidin markiertes Fusionsprotein.

Anwendung

Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physikalische Form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Angaben zur Herstellung

Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.

Rechtliche Hinweise

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Lagerklassenschlüssel

10 - Combustible liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Directed evolution of proteins in vitro using compartmentalization in emulsions.

Eric A Davidson et al.

Current protocols in molecular biology, Chapter 24, Unit 24-Unit 24 (2009-07-04)

This unit describes a protocol for the directed evolution of proteins utilizing in vitro compartmentalization. This method uses a large number of independent in vitro transcription and translation (IVTT) reactions in water droplets suspended in an oil emulsion to enable

MEK1 binds directly to betaarrestin1, influencing both its phosphorylation by ERK and the timing of its isoprenaline-stimulated internalization.

Dong Meng et al.

The Journal of biological chemistry, 284(17), 11425-11435 (2009-01-21)

betaArrestin is a multifunctional signal scaffold protein. Using SPOT immobilized peptide arrays, coupled with scanning alanine substitution and mutagenesis, we show that the MAPK kinase, MEK1, interacts directly with betaarrestin1. Asp(26) and Asp(29) in the N-terminal domain of betaarrestin1 are

The phosphorylation of Hsp20 enhances its association with amyloid-β to increase protection against neuronal cell death.

Ryan T Cameron et al.

Molecular and cellular neurosciences, 61, 46-55 (2014-05-27)

Up-regulation of Hsp20 protein levels in response to amyloid fibril formation is considered a key protective response against the onset of Alzheimer's disease (AD). Indeed, the physical interaction between Hsp20 and Aβ is known to prevent Aβ oligomerisation and protects

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