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Sigma-Aldrich

Jodnitrotetrazoliumchlorid

BioReagent, ≥97.0% (calc. on dry substance, NT)

Synonym(e):

2-(4-Iodphenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazoliumchlorid, p-Iodnitrotetrazolium violett, INT

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About This Item

Empirische Formel (Hill-System):
C19H13ClIN5O2
CAS-Nummer:
146-68-9
Molekulargewicht:
505.70
Beilstein:
4093224
EG-Nummer:
205-676-2
MDL-Nummer:
MFCD00011961
UNSPSC-Code:
12352102
PubChem Substanz-ID:
57651636
NACRES:
NA.25

Produktlinie

BioReagent

Qualitätsniveau

200

Assay

≥97.0% (calc. on dry substance, NT)

mp (Schmelzpunkt)

240 °C (dec.) (lit.)
~245 °C (dec.)

Löslichkeit

methanol: water (1:1): 50 mg/mL, very faintly turbid, very deep yellow (hot)

Anwendung(en)

histology

SMILES String

[Cl-].[O-][N+](=O)c1ccc(cc1)-[n+]2nc(nn2-c3ccc(I)cc3)-c4ccccc4

InChI

1S/C19H13IN5O2.ClH/c20-15-6-8-16(9-7-15)23-21-19(14-4-2-1-3-5-14)22-24(23)17-10-12-18(13-11-17)25(26)27;/h1-13H;1H/q+1;/p-1

InChIKey

JORABGDXCIBAFL-UHFFFAOYSA-M

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Anwendung

Iodonitrotetrazolium (INT) is a tetrazolium dye precursor that forms a purple formazan dye on reduction and has been used in a variety of applications. It is considered to have higher reactivity than some tetrazolium compounds, at least with respect to succinate dehydrogenase, with optimal results obtained using a concentration of 0.8 mM INT. INT is used as an electron acceptor for the colorimetric assays of: lactate dehydrogenase, xanthine dehydrogenase, lactyl-CoA dehydrogenase, succinate dehydrogenase, BBM II ketolisomerase, histidinol dehydrogenase and diverse other hydrolases.
Elektronenakzeptor für die kolorimetrische Untersuchung verschiedener Dehydrogenasen

Piktogramme

FlameExclamation mark
GHS02,GHS07

Signalwort

Danger

H-Sätze

H228,H335

Gefahreneinstufungen

Flam. Sol. 1 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Persönliche Schutzausrüstung

Eyeshields, Gloves, type N95 (US)

Analysenzertifikate (COA)

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Christopher Auger et al.
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Matthildi Valianou et al.
Scientific reports, 9(1), 3015-3015 (2019-03-01)
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Antoine Picciocchi et al.
The Journal of biological chemistry, 286(32), 28357-28369 (2011-06-11)
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Yang Hongwei et al.
Ecotoxicology and environmental safety, 59(2), 249-255 (2004-08-26)
Anaerobic biodegradability of organic compounds can be assessed from the changes in organic compounds, the end products, and the activity of microorganisms. In this study, all three of these variables were considered to integrate assessment of the anaerobic biodegradability of
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Biochemistry, 41(25), 8120-8133 (2002-06-19)
The properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803 were determined by means of kinetic and spectroscopic approaches in comparison to those exhibited by the bacterial NADPH-dependent enzyme form. The ferredoxin-dependent enzyme was found to be similar to
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