00185
Acetamid-Nährbouillon
suitable for microbiology, NutriSelect® Basic
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About This Item
Sterilität
non-sterile
Qualitätsniveau
Produktlinie
BioChemika
Form
powder
Haltbarkeit
limited shelf life, expiry date on the label
Hersteller/Markenname
NutriSelect® Basic
Methode(n)
microbe id | utilization test: suitable
pH-Endwert
7.0±0.2 (25 °C)
Anwendung(en)
bioburden testing
environmental
food and beverages
water monitoring
microbiology
Eignung
selective by defined nutrients for Pseudomonas spp.
Anwendung
Synthetisches Medium zur Detektion des mikrobiologischen Verbrauchs von Acetamid. Entspricht den Empfehlungen des "Deutschen Institut für Normung (DIN)" und den Testprozeduren aus §35 LMBG für die Untersuchung von Wasser auf Kontamination durch Pseudomonas aeruginosa.
Komponenten
Composition
Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L
Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L
Part A (Cat. No. 62192-109.4-KC)
Magnesium sulfate 0.158g/L
Sodium chloride 0.2g/L
Sodium molybdate 0.005g/L
Ferrous sulfate 0.0005g/L
Dipotassium hydrogen phosphate 0.2g/L
Part B (Cat. No. 00160-390.6G-KC)
Acetamide 2.0g/L
Angaben zur Herstellung
Directions:
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.
Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.
Dissolve 0.56 g Acetamide Nutrient Broth Part A and 2 g Acetamide in 950 ml distilled water. Check the pH and if necessary, adjust. Make up to 1 litre and pour into tubes. Autoclave at 121°C for 15 minutes.
Technique:
Medium is inoculated with a couple off loops from the culture to be assayed and it is incubated at 32-35°C for 24-48 hours before going to the isolation medium.
To confirm Ps. aeruginosa, inoculate a loop of culture in Asparagine Proline Broth (Cat. No. 17129) and incubate at 35-37°C for 24 hours. After this period, pour 1-2 drops of Nessler′s Reagent
(Cat. No. 72190) on to the culture and verify ammonia production: a change to yellow will mean ammonia production positive and therefore, Ps. aeruginosa presence.
Sonstige Hinweise
Decomposition of acetamide as taxonomic marker for some species of the genus Pseudomonas; Comparative studies on the enrichment of P. aeruginosa
Fußnote
We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.
Rechtliche Hinweise
GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany
Signalwort
Warning
H-Sätze
Gefahreneinstufungen
Carc. 2
Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Persönliche Schutzausrüstung
Eyeshields, Gloves, type P3 (EN 143) respirator cartridges
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R H Schubert et al.
Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie, 233(3), 342-346 (1975-11-01)
The comparative examination of the acetamide-decompostition and of the acetamide-utilisation as sole source of carbon demonstrates that various groups of the Pseudomonas species with the same kind of reaction have to be distinguished. The acetamide-decomposition-test according to BUHLMANN et al.
H.H. Guenich et al.
Zentralblatt Fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene, 252, 230-230 (1982)
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