S8005H
CpGenome 5-hmC DNA Standard
CpGenome 5-hmC DNA St&ard contains a linear, double-str&ed DNA (897 bp) with 100% hydroxymethylated cytosine. This st&ard can be used to calibrate applications aimed at analyzing & quantifying cytosine modifications.
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About This Item
UNSPSC-Code:
41105500
Empfohlene Produkte
Qualitätsniveau
Speziesreaktivität
human
Hersteller/Markenname
CpGenome
Upstate®
Versandbedingung
dry ice
Verwandte Kategorien
Allgemeine Beschreibung
CpGenome 5-hmC DNA Standard is a linear, double-stranded DNA (897 bp) containing 100% 5-hydroxymethylcytosine. This standard can be used to calibrate applications aimed at analyzing and quantifying cytosine modifications. This vial is a component of S8005 the CpGenome 5-mC and 5-hmC DNA Standard Set ( three linear, double-stranded DNAs (897 bp) with the same sequence, but containing either 100% 5-Methylcytosines (5-mC), 5-hydroxymethylcytosines (5-hmC), or unmodified cytosines.
Materials Provided:
One vial containing 2 µg (40 µL) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/μL.
Validation:
Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.
CpGenome is a trademark of Serologicals Corporation.
Materials Provided:
One vial containing 2 µg (40 µL) of CpGenome 5-hmC DNA Standard at a concentration of 50 ng/μL.
Validation:
Standards containing either 5-mC, 5-hmC, or unmodified cytosines were digested with 1 µL (20 units) of HpaII (which cleaves only unmethylated DNA), or PvuRts1I restriction enzyme (which targets only 5-hydroxymethylated DNA). The resulting DNA fragments were evaluated by agarose gel electrophoresis.
CpGenome is a trademark of Serologicals Corporation.
Anwendung
Research Category
Epigenetik & nukleäre Funktionen
Epigenetik & nukleäre Funktionen
Physikalische Form
Liquid in buffer containing 10 mM Tris-HCl, 1 mM EDTA, pH 8.0
Lagerung und Haltbarkeit
Recommended Storage: Stable for 6 months at -20°C from date of receipt.
Rechtliche Hinweise
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Haftungsausschluss
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Lagerklassenschlüssel
12 - Non Combustible Liquids
WGK
WGK 1
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Analysenzertifikate (COA)
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