PF060

TdT, Calf Thymus

• UNSPSC-Code: 12352202

Eigenschaften

• Form: liquid
• Qualitätsniveau: 100
• Enthält nicht: preservative
• Hersteller/Markenname: Calbiochem^®
• Lagerbedingungen: OK to freeze; avoid repeated freeze/thaw cycles
• Versandbedingung: wet ice
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Native TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxy terminus of either double of single stranded DNA in a template-independent manner.

Native TdT from calf thymus. Tdt is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3′ hydroxy terminus of either double of single stranded DNA in a template-independent manner. Although TdT preferentially adds deoxynucleotides to 3′-extensions, tailing onto 5′ overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, ³²P-dNTPs, cordycepin-dNTPs, or ddNTP.

The Calbiochem^® Terminal deoxynucleotidyl Transferase (TdT) is a DNA polymerase isolated from calf thymus. TdT catalyzes the addition of deoxynucleotides to the 3′-hydroxyl termini of either double or single stranded DNA molecules in a template independent manner. Although TdT preferentially adds deoxynucleotides to 3′-extensions, tailing onto 5′ overhangs or blunt ended double strands of DNA can also be achieved. TdT can be used for labeling DNA fragments and vectors with homopolymer tails and/or with modified deoxynucleotides such as biotin-dNTPs, 32P-dNTPs, cordycepin-dNTPs, or ddNTP.

Anwendung

DNA Labeling

Warnhinweis

Toxicity: Standard Handling (A)

Einheitendefinition

One unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3ʹ-OH terminus of d(A)50 in 60 min at 37°C.

Physikalische Form

775 Units in 50 mM potassium phosphate buffer, 1 mM B-mercaptoethanol, 50% glycerol, pH 7.2.

Rekonstituierung

Following initial thaw, aliquot and freeze (-20°C).

Sonstige Hinweise

Chang, L.M.S. and F.J. Bollum. 1986. Crit. Rev. Biochem.21, 27.
Deng, G. and R. Wu. 1983. Methods Enzymol. 100, 96.
Michelson, A.M. and S.H. Orkin. 1982. J. Biol. Chem.257, 14773.
Roychoudhury, R. and R. Wu. 1980. Methods Enzymol.65, 43.
Nelson, T. and D. Brutlag. 1979. Methods Enzymol. 68, 41.
Roychoudhury, R., et al. 1976. Nucleic Acids Res.3, 101.
Bollum, F.J. 1974. In The Enzymes, 3rd edition (ed. P.D. Boyer), Vol. 10, 145. Academic Press, New York.

Rechtliche Hinweise

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 2