MABN471

Anti-Tau Antibody, PAD, clone TNT-1

clone TNT-1, from mouse

• Synonym(e): Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau, Tau
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: mouse
• Qualitätsniveau: 100
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Klon: TNT-1, monoclonal
• Speziesreaktivität: mouse, rat, human
• Methode(n): ELISA: suitable; dot blot: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotyp: IgG1κ
• NCBI-Hinterlegungsnummer: NP_001116538
• UniProt-Hinterlegungsnummer: P10636
• Versandbedingung: wet ice
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... MAPT(4137) ; mouse ... Mapt(17762) ; rat ... Mapt(29477)

Beschreibung

Allgemeine Beschreibung

Tau or Microtubule-associated protein tau (MAPT), also known as neurofibrillary tangle protein and paired helical filament-tau (PHF-tau), is a cytosolic protein that promotes microtubule assembly and stability, and might be involved in the establishment and maintenance of axonal polarity in neurons. Tau binds to and is thought to function as a linker protein between axonal microtubules and neural plasma membrane components. There are multiple isoforms, and the short isoforms allow plasticity of the cytoskeleton whereas the longer isoforms may preferentially play a role in its stabilization. PAD is the phosphatase activating domain, and has been demonstrated to be involved in the inhibition of anterograde, kinesin-based fast axonal transport (FAT) by activating axonal protein phosphatase 1 (PP1) and glycogen synthase kinase 3 (GSK3), independent of microtubule binding. Defects in Tau are thought to be the cause of a number of neurodegenerative diseases, including frontotemporal dementia (FTD), pallido-ponto-nigral degeneration (PPND), Pick disease of the brain (PIDB), corticobasal degeneration (CBD), supranuclear palsy type 1 (PSNP1), Alzheimer disease, and Parkinson disease.

Immunogen

KLH-conjugated linear peptide corresponding to human Tau.

Anwendung

Research Category
Neurowissenschaft

Research Sub Category
Neurodegenerative Krankheiten

Anti-Tau, PAD, clone TNT-1 detects levels of Tau proteins & has been published & validated for use in WB, IHC, IF & ELISA.

Western Blot Analysis: 1 µg/mL from a representative lot detected Tau, PAD in 10 µg of human and mouse cortex tissue lysates.

Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Tau, PAD in human Alzheimer′s brain tissue.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Tau, PAD in normal and Alzheimer′s tissues from entorhinal cortex, hippocampus, inferior temporal gyrus, and superior gyrus of control (Kanaan, N. M., et al. (2011). J Neurosci. 31(27):9858-9868.).

ELISA Analysis: A representative lot from an independent laboratory detected Tau, PAD in recombinant Tau protein (Kanaan, N. M., et al. (2011). J Neurosci. 31(27):9858-9868.).

Qualität

Evaluated by Western Blot in rat cortex tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Tau, PAD in 10 µg of rat cortex tissue lysate.

Zielbeschreibung

~55 kDa observed. Many isoforms are known to exist between 35-80 kDa An uncharacterized band may be observed in some cell lysates.

Verlinkung

Replaces: MAB10417

Physikalische Form

Protein G Purified

Format: Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Lagerung und Haltbarkeit

Stable for 1 year at 2-8°C from date of receipt.

Hinweis zur Analyse

Control
rat cortex tissue lysate

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable