MABF1976M

Anti-Complement Receptor C5aR Antibody, clone 20/70

clone 20/70, from rat

• Synonym(e): C5a anaphylatoxin chemotactic receptor 1, C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88
• UNSPSC-Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rat
• Qualitätsniveau: 100
• Antikörperform: purified immunoglobulin
• Antikörper-Produkttyp: primary antibodies
• Klon: 20/70, monoclonal
• Speziesreaktivität: mouse
• Verpackung: antibody small pack of 25 μg
• Methode(n): activity assay: suitable; flow cytometry: suitable; immunocytochemistry: suitable; neutralization: suitable
• Isotyp: IgG2bκ
• NCBI-Hinterlegungsnummer: NP_031603.2
• UniProt-Hinterlegungsnummer: P30993
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: mouse ... C5Ar1(12273)

Beschreibung

Allgemeine Beschreibung

C5a anaphylatoxin chemotactic receptor 1 (UniProt: P30993; also known as C5a anaphylatoxin chemotactic receptor, C5a-R, C5aR, CD88) is encoded by the C5ar1 (also known as C5ar, C5r1) gene (Gene ID: 12273) in murine species. C5aR is a multi-pass membrane protein of the G-protein coupled receptor 1 family that serves as a receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release, and superoxide anion production. C5aR is a homodimer, but can also form higher-order oligomers. C5aR becomes phosphorylated on serine residues in response to C5a binding that results in internalization of the receptor and short-term desensitization to C5a. The phosphorylated receptor is known to Interact with arrestin beta 1 and 2 (ARRB1 and 2) and this interaction is associated with internalization of C5aR. Sulfation is also reported to play a critical role in the association of C5aR with C5a, but it does not alter the ability of the receptor to transduce a signal and mobilize calcium in response to a small peptide agonist.

Spezifität

Clone 20/70 detects complement 5a receptor in murine species. It targets an epitope with in the extracellular domain.

Immunogen

Rat basophilic leukemia RBL-2H3 cells stably expressing murine C5aR.

Anwendung

Anti-Complement Receptor C5aR, clone 20/70, Cat. No. MABF1976, is a rat monoclonal antibody that detects Complement 5a receptor (C5aR) and has been tested for use in Flow Cytometry, Activity Assay, Inhibition of Activity/Function, Immunocytochemistry, and Neutralizing.

Neutralizing Analysis: A representative lot neutralized Complement receptor C5aR (Godau, J., et. al. (2004). J Immunol. 173(5):3437-45).

Immunocytochemistry Analysis: A representative lot detected Complement receptor C5aR in Immunocytochemistry applications (Soruri, A., et. al. (2003). Immunol Lett. 88(1):47-52).

Inhibits Activity/Function Analysis: A representative lot inhibited Complement receptor C5aR in Activity/Function applications (Godau, J., et. al. (2004). J Immunol. 173(5):3437-45; Soruri, A., et. al. (2003).

Flow Cytometry Analysis: A representative lot detected Complement receptor C5aR in Flow Cytometry applications (Shushakova, N., et. al. (2002). J Clin Invest. 110(12):1823-30).

Activity Assay Analysis: A representative lot detected Complement receptor C5aR in Activity Assay applications (Shushakova, N., et. al. (2002). J Clin Invest. 110(12):1823-30).

Qualität

Evaluated by Flow Cytometry in BALB/c mouse bone marrow cells.

Flow Cytometry Analysis: 1 µg of this antibody detected Complement receptor C5aR in 1 million BALB/c mouse bone marrow cells.

Zielbeschreibung

39.02 kDa calculated.

Physikalische Form

Format: Purified

Sonstige Hinweise

Concentration: Please refer to lot specific datasheet.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable