ABE70-I

Anti-BAF180/PBRM1

• Synonym(e): Protein polybromo-1;hPB1;BRG1-associated factor 180;Polybromo-1D
• UNSPSC-Code: 12352203
• NACRES: NA.41

Eigenschaften

• Biologische Quelle: rabbit
• Qualitätsniveau: 200
• Antikörperform: purified antibody
• Antikörper-Produkttyp: primary antibodies
• Mol-Gew.: calculated mol wt 192.95 kDa; observed mol wt ~200 kDa
• Aufgereinigt durch: using Protein A
• Speziesreaktivität: human
• Speziesreaktivität (Voraussage durch Homologie): nonhuman primates
• Verpackung: antibody small pack of 100 μg
• Methode(n): western blot: suitable
• Isotyp: IgG
• Epitopsequenz: C-terminal half
• Protein-ID-Hinterlegungsnummer: NP_060783.3?
• UniProt-Hinterlegungsnummer: Q86U86
• Lagertemp.: 2-8°C
• Posttranslationale Modifikation Target: unmodified
• Angaben zum Gen: human ... PBRM1(55193)

Beschreibung

Allgemeine Beschreibung

Protein polybromo-1 (UniProt: Q86U86; also known as hPB1, BRG1-associated factor 180, BAF180, Polybromo-1D) is encoded by the PBRM1 (also known as BAF180, PB1) gene (Gene ID: 55193) in human. BAF180 is a widely expressed, bromodomain containing nuclear protein that is an essential component of the SWI/SNF-B (PBAF) chromatin remodeling complex. It is involved in transcriptional activation and repression of select genes by chromatin remodeling. It contains six bromodomains, two bromo-adjacent homology (BAH) domains (aa 956-1074 and 1156-1272), and one HMG-box (aa 1379-1447). The bromodomains recognize and bind to acetylated lysines and BAH domains mediate protein-protein interaction. Its HMG-box domain binds to DNA. BAF180 acts as a negative regulator of cell proliferation. It plays a role in DNA repair mechanisms and acts as a tumor suppressor. It is also involved in silencing transcription near double-stranded DNA breaks (DSB). The BAH domains of BAF180 are shown to be essential for ubiquitination of PCNA. BAF180 depleted cells can not produce proper levels of ubiquitinated PCNA that leads to the inability to replicate past DNA lesions. Mutations in PBRM1 gene have been linked to high incidence of renal cell carcinoma. Nine isoforms of BAF180 have been described that are produced by alternative splicing. (Ref.: Hopson, S., and Thompson, MJ. (2017). ACS Chem. Biol. 12(10); 2482-2490; Varela, I., et al. (2011). Nature. 469(7331); 539-542).

Spezifität

This rabbit polyclonal antibody specifically detects Protein polybromo-1 (BAF180). It targets an epitope within the C-terminal half.

Immunogen

GST-tagged recombinant fragment corresponding to 740 amino acids from the C-terminal half of human BAF180

Anwendung

Quality Control Testing

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Protein polybromo-1 (BAF180) in Jurkat cell lysate.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Physikalische Form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide

Rekonstituierung

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Lagerung und Haltbarkeit

Recommended storage: +2°C to +8°C.

Sonstige Hinweise

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Haftungsausschluss

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable