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HomePathogen & Spoilage TestingHybriScan® Rapid Microbial Test System

HybriScan® Rapid Microbial Test System

Rapid Microbiological Method: Sandwich Hybridization

The method simply needs a centrifuge, a thermal mixer and an optional a microplate reader. The complete analysis can be achieved in 2 hours for the HybriScan®D kit (quantitative kits) and about 1 hour for HybriScan®I kit (identification kits). Qualitative results can be determined by eye or, for quantitative measurement, use with a microplate reader.

HybriScan® system screening workflow

Figure 1.HybriScan® system screening workflow with analysis time of approx. 2–2.5 hours

Sensitivity, Specificity and Flexibility of Sandwich Hybridization

Sandwich hybridization is very sensitive and can detect attomoles of the target rRNA molecules in yeast or bacteria.1-3 These types of cells contain a large number of rRNA-containing ribosomes; a single cell therefore contains several thousand copies of rRNA but only one DNA copy. Sandwich hybridization provides sensitivity in crude biological samples because it is not susceptible to matrix interference. By using specific probes, the HybriScan® system allows both genus or species-specific detection and it is therefore applicable to many analytical fields, including monitoring the microbial content of beer, wine, non-alcoholic beverages, drinking water, a wide variety of foods and wastewater.

  • Detects viable microorganisms only—minimizes false-positive results from dead bacteria
  • Not sensitive to sample matrix
  • Genus- and species-specific detection
  • Results in just 2 hours after enrichment
  • Broad application range: beer, wine, non-alcoholic beverages, drinking water, food and wastewater

Advantages of HybriScan® technology over other microbial detection techniques

Detection TechnologyAdvantageDisadvantage
HybriScan® system
  • Detects only living cells 
  • Minimal interference by sample matrix
  • High specificity
  • Low cross-reactivity
  • Easy handling
  • Cost-efficient read-out
  • Quantitative and qualitative
  • High sample throughput (96-microwell plates)
  • Detects of non-culturable microbes
  • No differentiation of serotypes or subspecies
  • Limited probe design (rRNA target)
PCR
  • High sample throughput
  • Sensitive
  • Quantitative
  • No live/dead cell differentiation
  • Sensitive to matrix interference (high extraction effort)
  • Susceptible to polymerase inhibition
ELISA
  • Differentiation of serotypes or subspecies
  • High sample throughput (96-microwell plates)
  • Quantitative and qualitative
  • Low sensitivity
  • Low specificity, higher cross-reactivity
  • Slow and expensive assay development
Conventional cultivation-based methods
  • Relatively inexpensive
  • Simple
  • Specific
  • Widely accepted method
  • Time-consuming (up to 10 days)
  • No detection of non-culturable microbes
  • Low sample throughput
  • Laborious
HybriScan® technology

Figure 2.HybriScan® technology

HybriScan® Waste Water Bacterial Count Calculator

For our Wastewater Kits (04447 HybriScan® D Waste Water Microthrix parvicella, 78436 HybriScan® D Waste Water Total Bacterial Count), we provide an excel sheet (below) for calculation of total bacterial count, Microthrix count, and ratio of Microthrix: Total Bacterial Count.

Materials
Réf. du produitNom du produitDescriptionTarif
62533HybriScanD Beersuitable for microbiology
56917HybriScanD Campylobactersuitable for microbiology, Rapid test system for the detection of Campylobacter
12838HybriScanD Cronobacter spp.suitable for microbiology
68301HybriScanD Drinkssuitable for microbiology
96343HybriScanD E. colisuitable for microbiology
07190HybriScanD Legionella pneumophilasuitable for microbiology
16593HybriScanD Legionellasuitable for environmental, microbiology, suitable for microbe id | in situ hybriziation
49699HybriScanD Listeria monocytogenessuitable for microbiology
55662HybriScanD Salmonellasuitable for microbiology
02349HybriScanD Total Bacterial Countsuitable for microbiology
04447HybriScanD Waste Water Microthrix parvicellasuitable for microbiology
78436HybriScanD Waste Water Total Bacterial Countsuitable for environmental, microbiology, suitable for microbe id | in situ hybriziation
61397HybriScanD Yeastsuitable for microbiology
39851HybriScanI Alicyclobacillussuitable for microbiology
79742HybriScanI Brettanomycessuitable for food and beverages, microbiology, suitable for microbe id | in situ hybriziation
19503HybriScan I Candida albicanssuitable for microbiology
76545HybriScanI E. colisuitable for microbiology
75724HybriScanI Lactobacillus brevissuitable for food and beverages, microbiology, suitable for microbe id | in situ hybriziation
49417HybriScanI Legionella pneumophilasuitable for environmental, microbiology, suitable for microbe id | in situ hybriziation
77007HybriScanI Leuconostocsuitable for food and beverages, microbiology, suitable for microbe id | in situ hybriziation

References

1.
Huhtamella S, Leinonen M, Nieminen T, Fahnert B, Myllykoski L, Breitenstein A, Neubauer P. 2007. RNA-based sandwich hybridisation method for detection of lactic acid bacteria in brewery samples. Journal of Microbiological Methods. 68(3):543-553. https://doi.org/10.1016/j.mimet.2006.10.009
2.
Leskelä T, Tilsala-Timisjärvi A, Kusnetsov J, Neubauer P, Breitenstein A. 2005. Sensitive genus-specific detection of Legionella by a 16S rRNA based sandwich hybridization assay. Journal of Microbiological Methods. 62(2):167-179. https://doi.org/10.1016/j.mimet.2005.02.008
3.
Rautio J, Barken K, Lahdenperä J, Breitenstein A, Molin S, Neubauer P. 2003. Microb Cell Fact. 2(1):4. https://doi.org/10.1186/1475-2859-2-4
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