Biopolymer Separations

HPLC Columns and Chromatographic Media for the Analysis and Purification of Biopolymers

Biopolymers, including peptides, proteins, polynucleotides, and other biodegradable polymers, vary in terms of net ionic charge, hydrophobicity, size, shape and mass. In addition to these structural (and functional) complexities, an individual biopolymer is typically present at low concentration in samples that contain many similar and similarly complex compounds. Thus, peptides, proteins, and polynucleotides are often analyzed, isolated and purified by multiple modes of chromatography, including size-exclusion, ion-exchange, reversed phase, hydrophobic interaction, hydrophilic interaction, and/or affinity chromatography.

We offer a wide variety of stationary phases for the analysis and purification of large biomolecules, developed and manufactured by Supelco or in cooperation with our technology partners. In addition, we offer columns from well-known brands, including Tosoh Bioscience, Cytiva, Sepax^® Technologies and Kromasil.

Our portfolio of U/HPLC and FPLC columns for biopolymer separations is organized by mode of chromatography, and by column brand or manufacturer. Recent additions to the product portfolio include BIOshell™ Glycan columns to determine the glycan composition of glycoproteins, gel-filtration and ion-exchange columns from Sepax^® Technologies, and the complete line of TSKgel^® HPLC columns and Toyopearl^® media and process development columns from Tosoh Bioscience.

Bookmark this page as we continue to add new applications, technical papers and exciting separation tools to advance your biopolymer research.

Technical Resources

BIOshell™
BIOshell™ Fused-Core® Columns (T414048)	Brochure
BIOshell™ Guard Columns (T413198)	Product Info.
BIOshell™ Fused-Core® Columns for Ultra Efficient RPLC of Proteins and Peptides: Analysis of IgG fragments (T413210)	Product Info.
Empowering the Biochemist to Analyze Glycoproteins with Exceptional Reproducibility	Technical Report
Faster Protein and Peptide Liquid Chromatography: Introducing BIOshell™ Fused-Core® HPLC Columns (Reporter 32.1)	Technical Report
Care and Use Guides
2.0 µm BIOshell™ A160 Peptide C18 Columns, 2.0 µm
2.7 µm BIOshell™ A160 Peptide C18 Columns, 2.7 µm BIOshell™ A160 Peptide CN Columns, 2.7 µm BIOshell™ A160 Peptide Phenyl-Hexyl Columns, 2.7 µm BIOshell™ Glycan Columns, 2.7 µm BIOshell™ IgG 1000 Å C18 Columns, 2.7 µm BIOshell™ IgG 1000 Å C4 Columns, 2.7 µm BIOshell™ IgG 1000 Å Diphenyl Columns, 2.7 µm BIOshell™ A160 Peptide CN Nano/Capillary Columns, 2.7 µm
3.4 µm BIOshell™ A400 Protein C18 Columns, 3.4 µm BIOshell™ A400 Protein C4 Columns, 3.4 µm BIOshell™ A400 Protein C18 Nano/Capillary Columns, 3.4 µm BIOshell™ A400 Protein C4 Nano/Capillary Columns, 3.4 µm
5.0 µm BIOshell™ A160 Peptide C18 Columns, 5.0 µm BIOshel ™ A160 Peptide CN Columns, 5.0 µm BIOshell™ A160 Peptide CN Nano/Capillary Columns, 5.0 µm

TSKgel®
TSKgel® Gel Filtration Columns, PW type (T494075)	Product Info.
TSKgel® Gel Filtration Column, SW type (T494076)	Product Info.
TSKgel® Ion Exchange Columns (T494077)	Product Info.
TSKgel® Hydrophobic Interaction Columns (T494078)	Product Info.
TSKgel® Reversed Phase Columns (T494079)	Product Info.
TSKgel® Affinity Columns (T494080)	Product Info.
TSKgel® Nonporous Resin Columns for Rapid Analysis of Proteins, Peptides, and Nucleic Acids - Bulletin 862 (T109862)	Technical Report
Innovative Approach to Coffee Bean Characterization, Using SEC and High Resolution - Bulletin 919 (T197919)	Technical Report

Discovery
Discovery® Wide Pore HPLC Columns for Biomolecule separation	Brochure
Discovery BIO PolyMA: Ion exchange columns for protein and peptide separations (T410079)	Brochure
Discovery BIO PolyMA: Ion exchange columns for protein and peptide separations (T403118)	Poster
Enhanced Stability of Discovery BIO Wide Pore C5 Compared to Other Short Alkyl Chain Phases (T103935)	Bulletin 935
Getting Started with RP-HPLC for Peptides (T302166)	App Note 166
Increase Sensitivity and Decrease Sample Consumption Using HPLC Microbore and Capillary Column Dimensions (T302167)	App Note 167
Eliminate TFA and Improve Sensitivity of Peptide Analyses by LC/MS (T302168)	App Note 168
Effect of Stationary Phase on Selectivity of Reversed-Phase HPLC Separations of Polypeptides (T302169)	App Note 169
Peptide Separations on a Stable, Aqueous Compatible C18 (T403056)	Technical Report

Sepax®
Separation of Polysaccharides on SRT®	App Note
Size Exclusion Separation of Protein Mixtures: Comparison of SRT® Columns and TSKgel® Columns	App Note
Analysis of MAb 221 with Tween 80 and Tween 20	App Note
Analysis of Vaccine Sample by Sepax®SRT SEC-2000 Column	App Note
PEG Separation on Zenix® SEC	App Note
Oligonucleotide Separation on Zenix® SEC and Proteomix® SAX	App Note
Zenix® SEC 7.8x200mm	App Note
Fusion Protein Separation	App Note
Intact Glycoprotein Isoforms Separation on Proteomix® SAX Chromatography	App Note
Monoclonal Antibody Fragment Separation Using SEC Coupled with M-S	App Note
BSA pegylation	App Note
Analysis of Glycoproteins on Proteomix® SAX-NP10	App Note
Analysis of MAb 321 on Proteomix® SCX NP5	App Note
Analysis of mAb using a pH Gradient on Proteomix® SCX NP5	App Note
Antibodix® User Manual	Brochure
Proteomix®User Manual	Brochure
SRT® SEC User Manual	Brochure
SRT®-C SEC User Manual	Brochure
Zenix® User Manual	Brochure
Zenix® -C User Manual	Brochure

Kromasil
Kromasil 300 Å for your Protein Separations with Kromasil HPLC Columns (T409220)	Product Info.
Kromasil 300 Å - SIL, C4, C8, C18 (T409221)	Product Info.