HomeSmall Molecule HPLCUHPLC/MS Analysis of Cannabinoids Extracted from Raw Cannabis Sativa (Marijuana) on Ascentis® Express RP-Amide
UHPLC/MS Analysis of Cannabinoids Extracted from Raw Cannabis Sativa (Marijuana) on Ascentis® Express RP-Amide
Conditions
Sample Preparation | A 100 mg sample of dried cannabis sativa leaves was extracted with 50 mL of ethanol:water (50:50, v/v) for 15 mins in an ultrasonic bath. (The sample was then filtered through a 0.45 µM Millex® LH filter prior to LC/MS analysis.) |
Column | Ascentis® Express RP-Amide, 10 cm x 2.1 mm I.D., 2.0 µm particles (53913-U) |
Column Temp. | 35 °C |
Mobile phase | [A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile |
Gradient | 60% B to 100% B in 10 min |
Flow Rate | 0.4 mL/min |
Pressure | 7600 psi (524 bar) |
Injection | 1 µL |
Detector | MS, ESI(+), ESI(-), MRM |
Description
Analysis Note | Cannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, inflammatory diseases, and other health conditions. Shown here is a raw cannabis sample extracted using a simple procedure followed by LC/MS/MS analysis. Eight major cannabis compounds were detected. Reversed-phase HPLC separation was achieved on an Ascentis Express RP-Amide column. Dried cannabis sample was obtained courtesy of Dr. Hari H. Singh, Program Director at the Chemistry & Physiological Systems Research Branch of the National Institute on Drug Abuse at the US National Institute of Health. The extract strain of the sample was not known. |
Categories | Analytical Chromatography, Pharmaceuticals, Herbal medicines, Drugs of Abuse |
Featured Industry | Food and Beverages Forensics and Toxicology Vitamins, Nutraceuticals, and Natural Products |
Legal Information | Ascentis is a registered trademark of Sigma-Aldrich Co. LLC |
Suitability | Application for UHPLC-MS |
Materials
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