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HomeSmall Molecule HPLCUHPLC/MS Analysis of Cannabinoids Extracted from Raw Cannabis Sativa (Marijuana) on Ascentis® Express RP-Amide

UHPLC/MS Analysis of Cannabinoids Extracted from Raw Cannabis Sativa (Marijuana) on Ascentis® Express RP-Amide

UHPLC/MS Analysis of Cannabinoids Extracted

Conditions

Sample PreparationA 100 mg sample of dried cannabis sativa leaves was extracted with 50 mL of ethanol:water (50:50, v/v) for 15 mins in an ultrasonic bath. (The sample was then filtered through a 0.45 µM Millex® LH filter prior to LC/MS analysis.)
ColumnAscentis® Express RP-Amide, 10 cm x 2.1 mm I.D., 2.0 µm particles (53913-U)
Column Temp.35 °C
Mobile phase[A] 0.1% formic acid; [B] 0.1% formic acid in acetonitrile
Gradient60% B to 100% B in 10 min
Flow Rate0.4 mL/min
Pressure7600 psi (524 bar)
Injection1 µL
DetectorMS, ESI(+), ESI(-), MRM

Description

Analysis NoteCannabis compounds reportedly have therapeutic efficacy in the treatment of pain, mood disorders, inflammatory diseases, and other health conditions. Shown here is a raw cannabis sample extracted using a simple procedure followed by LC/MS/MS analysis. Eight major cannabis compounds were detected. Reversed-phase HPLC separation was achieved on an Ascentis Express RP-Amide column. Dried cannabis sample was obtained courtesy of Dr. Hari H. Singh, Program Director at the Chemistry & Physiological Systems Research Branch of the National Institute on Drug Abuse at the US National Institute of Health. The extract strain of the sample was not known.
CategoriesAnalytical Chromatography, Pharmaceuticals, Herbal medicines, Drugs of Abuse
Featured IndustryFood and Beverages
Forensics and Toxicology
Vitamins, Nutraceuticals, and Natural Products
Legal InformationAscentis is a registered trademark of Sigma-Aldrich Co. LLC
SuitabilityApplication for UHPLC-MS
Materials
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