HPLC Analysis of Penicillin G and Phenoxymethylpenicillinic Acid on Ascentis^® C18

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

[A] 10 mM ammonium acetate (pH 4.5 acetic acid): [B] acetonitrile, (75:25, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL

sample

100 μg/mL each in mobile phase