HPLC Analysis of Verapamil and Metabolites in Rat Plasma on Ascentis^® Express C18 after Phospholipid Removal using HybridSPE^®-Phospholipid

SPE well plate

HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)

sample preparation

SPE (Solid Phase Extraction)

column

Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)

sample/matrix

Rat plasma spiked with verapamil and metabolites at 10 ng/mL

mobile phase

[A] water; [B] acetonitrile, pH 2.55 adjust with formic acid (30:70, A:B)

flow rate

0.6 mL/min

column temp.

35 °C

detector

TOF/MS

injection

1 μL

sample addition

100 μL spiked rat plasma followed by 300 μL 1% formic acid in acetonitrile

elution

apply vacuum