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HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18

HPLC Analysis of Vitamin D2 and Vitamin D3 on Ascentis® C18 application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

581324-U

Colonne Ascentis® C18 pour HPLC

5 μm particle size, L × I.D. 15 cm × 4.6 mm

Étalon

Réf. du produit
Description
Tarif

47763

Cholecalciferol (D3)

analytical standard

47768

Ergocalciferol (D2)

analytical standard

CONDITIONS

column

Ascentis C18, 15 cm x 4.6 mm I.D., 5 μm particles (581324-U)

mobile phase

acetonitrile

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 290 nm

sample

50 μg/mL each in acetonitrile

Description

Remarque sur l'analyse

This application demonstrates the suitability of the Ascentis C18 for the analysis of the fat-soluble vitamins D2 and D3 . Structures along with the optimized chromatogram obtained on the Ascentis C18 are presented.

Informations légales

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS