HPLC Analysis of Vitamin D2 and D3 on Ascentis® Express C18
CONDITIONS
column
Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)
mobile phase
acetonitrile
flow rate
0.15 mL/min
column temp.
35 °C
detector
UV, 290 nm
injection
10 μL
sample
10 mg/L each (vitamins D2 and D3) in methanol
Description
Informations légales
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany