HPLC Analysis of Norleucine Enantiomers on Astec^® CHIROBIOTIC^® T

column

CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] ethanol; (80:20, A:B)

flow velocity

1 mL/min

column temp.

25 °C

detector

UV, 210 nm

injection

5 μL

sample

5 mg/mL in methanol