HPLC Analysis of Benzene and Deuterated Benzene on Ascentis^® Express C18

column

Ascentis Express C18, 15 cm × 4.6 mm I.D., 2.7 μm particles (53829-U)

mobile phase

[A] acetonitrile; [B] water (55:45, A:B)

flow rate

1.0 mL/min

column temp.

50 °C

detector

UV, 254 nm

injection

10 μL