HPLC Analysis of Amides on Discovery^® RP-Amide C16

column

Discovery RP-Amide C16, 15 cm × 4.6 mm, 5 μm particles (505013)

mobile phase

[A] methanol; [B] 25mM monopotassium phosphate, pH 3.0; (20:80, A:B)

flow rate

2 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

10 μL