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HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell Glycan using HILIC-FLR

HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell Glycan using HILIC-FLR application for HPLC

Matériaux

Colonne analytique

Réf. du produit
Description
Tarif

BIOshell Glycan HPLC Columns

L × I.D. 15 cm × 2.1 mm, 2.7 μm particle size

CONDITIONS

column

HPLC BIOshell Glycan Column, 15 cm x 2.1 mm I.D., 2.7 μm particles (50994-U)

mobile phase

A: 50 mM ammonium formate (CAt. No. 17843) pH 4.4, [ammonium] = 50 mM, pH adjust with formic acid (Fluka 94318) B: Acetonitrile (Sigma-Aldrich 34851)

gradient

75% to 59% B in 75 min

flow rate

0.3 mL/min

detector

FLR (308 nm excitation, 359 emission)

injection

10 μL

sample

Dextran Ladder (31417) after Procainamide Labeling Protocol

Description

Remarque sur l'analyse

The dextran ladder is used as an external standard for the analysis of glycans by normal phase HPLC after fluorescent labeling. When analyzed on the BIOshell Glycan HPLC Column, this standard gives a characteristic ladder profile from monomeric glucose to approximately a 20-mers of glucose oligosaccharide. This ladder provides calibration reference points that can aide in identifying more complex glycans based upon retention characteristics.

Informations légales

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