- Validation of a method for sedatives and β-blockers determination in swine, bovine and equine kidney using liquid chromatography coupled with tandem mass spectrometry.
Validation of a method for sedatives and β-blockers determination in swine, bovine and equine kidney using liquid chromatography coupled with tandem mass spectrometry.
Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & risk assessment (2016-11-22)
Lenise Guimarães de Oliveira, Fabiano Barreto, Rodrigo Hoff, Gabriel Rübensam, Marcia Helena Scherer Kurz, Gabriela Galle, Fábio Ferreira Gonçalves
PMID27869552
RÉSUMÉ
A method for the determination of five sedatives and 14 β-blocker residues in swine, bovine and equine kidney was validated. Samples were extracted with acetonitrile and purified using dispersive solid phase extraction (d-SPE) with Celite 545 with subsequent analysis by LC-MS/MS. A simplified protocol was applied to validate the method scope extension to include new matrices. Parameters such as recovery, trueness, linearity (r