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Bioluminescent liquid light guide pad biosensor for indoor air toxicity monitoring.

Analytical chemistry (2015-03-17)
Evgeni Eltzov, Avital Cohen, Robert S Marks
RÉSUMÉ

Indoor air pollution became a recent concern found to be oftentimes worse than outdoor air quality. We developed a tool that is cheap and simple and enables continuous monitoring of air toxicity. It is a biosensor with both a nondisposable (monitor) and disposable (calcium alginate pads with immobilized bacteria) elements. Various parameters to enhance its signal have been tested (including the effect of the pad's orientation, it's exposure to either temperature or time with the air toxicant analyte, and various concentrations thereof). Lastly, the sensor has demonstrated its ability to sense the presence of chemicals in a real, indoor environment. This is the first step in the creation of a sensitive and simple operative tool that may be used in different indoor environments.

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Sigma-Aldrich
Méthanol, suitable for HPLC, ≥99.9%
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Acétone, ACS reagent, ≥99.5%
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Tetrahydrofurane, inhibitor-free, suitable for HPLC, ≥99.9%
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Méthanol, ACS reagent, ≥99.8%
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Hydroxyde d′ammonium solution, ACS reagent, 28.0-30.0% NH3 basis
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Acétone, suitable for HPLC, ≥99.9%
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N,N-Diméthylformamide, ACS reagent, ≥99.8%
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N,N-Diméthylformamide, suitable for HPLC, ≥99.9%
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Méthanol, suitable for HPLC, gradient grade, ≥99.9%
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Acétone, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
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Toluène, ACS reagent, ≥99.5%
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Chloroforme, contains 100-200 ppm amylenes as stabilizer, ≥99.5%
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Tetrahydrofurane, contains 250 ppm BHT as inhibitor, ACS reagent, ≥99.0%
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N,N-Diméthylformamide, anhydrous, 99.8%
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Éther diéthylique, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor
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Pyridine, ACS reagent, ≥99.0%
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Chloroforme, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%, contains amylenes as stabilizer
Sigma-Aldrich
Méthanol, HPLC Plus, ≥99.9%
Sigma-Aldrich
Éther diéthylique, suitable for HPLC, ≥99.9%, inhibitor-free
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Toluène, suitable for HPLC, 99.9%
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Chloroforme, suitable for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
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Toluène, HPLC Plus, for HPLC, GC, and residue analysis, ≥99.9%
Sigma-Aldrich
Toluène, anhydrous, 99.8%
Sigma-Aldrich
Éther diéthylique, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor