Accéder au contenu
MilliporeSigma

Thermodynamic and structural characterization of the specific binding of Zn(II) to human protein DJ-1.

Biochemistry (2014-04-05)
Shinya Tashiro, Jose M M Caaveiro, Chun-Xiang Wu, Quyen Q Hoang, Kouhei Tsumoto
RÉSUMÉ

Mutations of DJ-1 cause familial Parkinson's disease (PD), although the role of DJ-1 in PD remains unresolved. Very recent reports have shown that DJ-1 interacts with copper ions. This evidence opens new avenues to understanding the function of DJ-1 and its role in PD. Herein, we report that Zn(II) binds to DJ-1 with great selectivity among the other metals examined: Mn(II), Fe(II), Co(II), Ni(II), and Cu(II). High-resolution X-ray crystallography (1.18 Å resolution) shows Zn(II) is coordinated to the protein by the key residues Cys106 and Glu18. These results suggest that DJ-1 may be regulated and/or stabilized by Zn(II).

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Zinc, dust, <10 μm, ≥98%
Sigma-Aldrich
Zinc, powder, <150 μm, 99.995% trace metals basis
Sigma-Aldrich
Zinc, nanopowder, 40-60 nm avg. part. size, ≥99% trace metals basis
Sigma-Aldrich
Zinc, foil, thickness 0.25 mm, 99.9% trace metals basis
Sigma-Aldrich
Zinc, granular, 20-30 mesh, ACS reagent, ≥99.8%
Zinc, foil, 300x300mm, thickness 0.1mm, as rolled, 99.95+%
Sigma-Aldrich
Zinc, granular, 30-100 mesh, 99%
Sigma-Aldrich
Zinc preparation, 5 g/dL Zn+2 in THF, highly reactive Rieke®metal
Sigma-Aldrich
Zinc, sticks, diam. 7-10 mm, 99.97% trace metals basis
Sigma-Aldrich
Zinc, mossy, ≥99%
Sigma-Aldrich
Zinc, purum, powder
Sigma-Aldrich
Zinc, shot, <12 mm, 99.99% trace metals basis
Sigma-Aldrich
Zinc, shot, 5 mm, 99.999% trace metals basis
Sigma-Aldrich
Zinc, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.9%, granular
Sigma-Aldrich
Zinc, foil, thickness 0.25 mm, 99.999% trace metals basis
Sigma-Aldrich
Zinc, foil, thickness 1.0 mm, 99.99% trace metals basis
Sigma-Aldrich
Zinc, pieces, 2-14 mesh, 99.9% trace metals basis
Sigma-Aldrich
Zinc, wire, diam. 1.0 mm, 99.995% trace metals basis
Zinc, wire reel, 5m, diameter 1.0mm, 99.99+%
Zinc, foil, 100x100mm, thickness 0.15mm, as rolled, 99.95+%
Zinc, foil, 100x100mm, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 50x50mm, thickness 0.1mm, as rolled, 99.95+%
Zinc, foil, 0.5m coil, thickness 0.05mm, as rolled, 99.95+%
Zinc, wire reel, 5m, diameter 2.0mm, 99.99+%
Zinc, wire reel, 0.1m, diameter 1.0mm, 99.999%
Zinc, foil, 50x50mm, thickness 0.15mm, as rolled, 99.95+%
Zinc, foil, 1m coil, thickness 0.025mm, as rolled, 99.95+%
Zinc, foil, 150x150mm, thickness 0.15mm, as rolled, 99.95+%
Zinc, foil, 50mm disks, thickness 0.175mm, as rolled, 99.95+%
Zinc, foil, 25x25mm, thickness 3mm, as rolled, 99.99+%