- An improved method for labeling carboxyatractyloside by [3H]KBH4.
An improved method for labeling carboxyatractyloside by [3H]KBH4.
Biochemistry international (1984-02-01)
S Ishiyama, K Hiraga, S Tuboi
PMID6477602
RÉSUMÉ
Carboxyatractyloside was labeled with [3H]KBH4 after oxidation of the primary alcohol of the glucose disulfate moiety by dicyclohexylcarbodiimide and P2O5 under anhydrous conditions in a dimethylsulfoxide medium. The 3H-labeled product was purified by DE 52 column chromatography followed by Cellulofine GCL 25 column chromatography. The final 3H-labeled product gave a single spot on a thin-layer chromatogram, and its Rf value was the same as that of authentic carboxyatractyloside. The biological activities (such as inhibition of state 3 respiration and binding to the adenine nucleotide carrier) were also comparable with those of authentic carboxyatractyloside.