Accéder au contenu
MilliporeSigma

Development of a benzophenone and alkyne functionalised trehalose probe to study trehalose dimycolate binding proteins.

Organic & biomolecular chemistry (2012-12-22)
Ashna A Khan, Faustin Kamena, Mattie S M Timmer, Bridget L Stocker
RÉSUMÉ

Trehalose dimycolates (TDMs) are the most abundant glycolipids found in the cell wall of Mycobacterium tuberculosis (M. tb). TDMs play an important role in the pathogenesis of M. tb yet the only known receptor for TDM is the macrophage inducible C-type lectin (mincle). To understand more about the interaction of TDMs with immune cells, affinity based proteome profiling (AfBPP) can be used to determine receptors that bind TDMs. To this end, we present the synthesis of the first AfBPP-TDM probe and report on its ability to activate macrophages. By doing so, we establish that the AfBPP-TDM probe appears to be a suitable substrate for future proteomic profiling experiments.

MATÉRIAUX
Référence du produit
Marque
Description du produit

Sigma-Aldrich
Benzophénone, ReagentPlus®, 99%
Sigma-Aldrich
Benzophénone, purified by sublimation, ≥99%
Supelco
Benzophénone, analytical standard
Supelco
Benzophénone, Pharmaceutical Secondary Standard; Certified Reference Material
Supelco
Benzophénone, traceable to primary standards (LGC)
Supelco
Melting point standard 47-49°C, analytical standard
Benzophénone, European Pharmacopoeia (EP) Reference Standard